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ZRB2157

Sigma-Aldrich

Anti-MTA1 Antibody, clone 1G22 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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Synonym(s):
Metastasis-associated protein MTA1

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1G22, recombinant monoclonal

description

1G22 Clone

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 80.79 kDa
observed mol wt ~80 kDa

purified by

using Protein A

species reactivity

human, rat, mouse

species reactivity (predicted by homology)

monkey, guinea pig

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

epitope sequence

C-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... MTA1(9112)

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ZRB1636ZRB1576ZRB1528
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

clone

1G22, recombinant monoclonal

clone

1G2, recombinant monoclonal

clone

1H15, recombinant monoclonal

clone

1L13, recombinant monoclonal

Gene Information

human ... MTA1(9112)

Gene Information

-

Gene Information

-

Gene Information

human ... BICRAL(23506)

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1G22 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Metastasis-associated protein MTA1. It targets an epitope within 25 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human Metastasis-associated protein MTA1.

Application

Quality Control Testing

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MTA1 in Raji cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MTA1 in lysates from HeLa and C2C12 cells and Rat brain tissue.

Affinity Binding Assay: A representative lot of this antibody bound MTA1 peptide with a KD of 1.3 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MTA1 in human testis tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Metastasis-associated protein MTA1 (UniProt: Q13330; also known as MTA1) is encoded by the MTA1 gene (Gene ID: 9112) in human. MTA1 is a component of the nucleosome-remodeling and histone-deacetylase multiprotein complex (NuRD). It is widely expressed with high expression observed in brain, liver, kidney, and cardiac muscle, ovaries, adrenal glands, and virgin mammary glands. It serves as a transcriptional coregulator that can act as both a transcriptional corepressor and coactivator. As a part of the NuRD complex, it regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA. In conjunction with other components of NuRD, it acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A. It also acts as a transcriptional coactivator of BCAS3, PAX5 and SUMO2, independent of the NuRD complex. MTA1 plays an important role in tumorigenesis, tumor invasion, and metastasis. It is up-regulated in a wide variety of cancers including breast, liver, ovarian, and colorectal cancer, and its expression levels are closely correlated with tumor aggressiveness and metastasis. MTA1s is phosphorylated by CKI- 2 and that enhances its ability to sequester ER- in the cytoplasm. A short and a long isoform of MTA1 have been described. The short isoform is cytosolic and contains a Leu-Arg-Ile-Leu-Leu (ER binding) motif. The long isoform is present in the nucleus, nuclear envelope, and in the cytosol. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gururaj, AE., et al. (2006). Proc. Natl. Acad. Sci. USA.103(17); 6670-6675; Mishra, SK., et al. (2004). Oncogene. 23(25); 4422-4429).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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