ZRB2271

Anti-SMAD7 Antibody, clone 2D15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Anti-IL-13 Antibody, clone 1B10 ZooMAb^® Rabbit Monoclonal, MAD homolog 7, MAD homolog 8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, Mothers against decapentaplegic homolog 7, Mothers against decapentaplegic homolog 8, SMAD family member 7, hSMAD7
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2D15, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 46.43 kDa; observed mol wt ~40 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): mouse, rat
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_005895
• UniProt accession no.: O15105
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... SMAD7(4092)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2D15 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects SMAD7. It targets an epitope within 24 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human SMAD7.

Application

Quality Control Testing

Evaluated by Western Blotting in SH-SY6Y cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SMAD7 in SH-SY5Y cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SMAD7 in A549 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SMAD7 in U-251 MG cells.

Affinity Binding Assay: A representative lot of this antibody bound SMAD7 with a KD of 1.2 x 10^-6 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SMAD7 in Human kidney tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Mothers against decapentaplegic homolog 7 (UniProt: O15105; also known as MAD homolog 7, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 8, Mothers against DPP homolog 8, SMAD family member 7, SMAD 7, Smad7, hSMAD7) is encoded by the SMAD7 (also known as MADH7, MADH8) gene (Gene ID: 4092) in human. At least nine SMAD proteins have been described in vertebrates that are classified as: (a) receptor-activated SMADs (R-SMADs), (b) co-mediator SMADs, and (c) inhibitory SMADs (I-SMADs). SMAD6 and 7 belong to the group of I-SMADs. SMAD7 is ubiquitously expressed protein with higher expression observed in the lung and vascular endothelium. It is a key negative regulator of TGF-β signaling and plays an important role in modulating a large array of biological processes. It antagonizes TGF-β signaling through multiple mechanisms, such as by interfering with the recruitment of R-SMADs upon TGF-β/activin activation, by inducing degradation of TGF-β type I r

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable