ZRB2974
Anti-cPLA2 Antibody, clone 2F4 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Cytosolic phospholipase A2, Phospholipase A2 group IVA
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2F4, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 85.24 kDa
observed mol wt ~100 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
Internal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... PLA2G4A(5321)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2F4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects cytosolic phospholipase A2 (cPLA2). It targets an epitope within 23 amino acids from the internal region.
Immunogen
KLH-conjugated linear peptide corresponding to 23 amino acids from the internal region of human cytosolic Phospholipase A2 (cPLA2).
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cPLA2 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected cPLA2 in lysates from A549 cells and Human testis tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected cPLA2 in Human kidney and 1:100 dilution in Human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound cPLA2 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected cPLA2 in HeLa cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected cPLA2 in lysates from A549 cells and Human testis tissue.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected cPLA2 in Human kidney and 1:100 dilution in Human testis tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound cPLA2 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Cytosolic phospholipase A2 (UniProt: P47712; also known as cPLA2, Phospholipase A2 group IVA) is encoded by the PLA2G4A (also known as CPLA2, PLA2G4) gene (Gene ID: 5321) in human. cPLA2 is a calcium-dependent phospholipase with lysophospholipase activities. However, it also displays calcium-independent phospholipase A2 and lysophospholipase activities in the presence of phosphoinositides. It preferentially hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids. Its expression is observed in various cells and tissues, including macrophages, neutrophils, fibroblasts, and lung endothelium. It is detected in cytoplasm, nucleus, and Golgi apparatus membranes. It plays a major role in membrane lipid remodeling and biosynthesis of lipid mediators of the inflammatory response. It also plays a role in embryo implantation and parturition through its ability to trigger prostanoid production. It is reported to selectively hydrolyze sn-2 arachidonoyl group from membrane phospholipids, thereby providing the precursor for eicosanoid biosynthesis via the cyclooxygenase pathway. cPLA2 undergoes phosphorylation at serine 505 and serine 727 by MAP kinases in response to mitogenic stimuli. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: (Ref.: Six, DA., and Dennis EA. (2003). J. Biol. Chem. 278(26); 23842-23850; Hanel, AM., and Gelb, MH. (1995). Biochemistry. 34(24); 7807-7818; Lin, LL., et al. (1993). Cell. 72(2); 269-278).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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