ZRB2993

Anti-CD83 Antibody, clone 1I15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Cell surface protein HB15, hCD83
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1I15, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 23.04 kDa; observed mol wt ~39 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_004224
• UniProt accession no.: Q01151
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CD83(9308)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1I15 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CD83. It targets an epitope within 20 amino acids from the extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the extracellular domain of human CD83.

Application

Quality Control Testing

Evaluated by Western Blotting with His-tagged, recombinant fragment of human CD83.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant fragment of human CD83.

Tested Applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected CD83 in Raji cell lysate.

Affinity Binding Assay Analysis: The binding affinity of this antibody to CD83 peptide was at least one thousand-fold (1,000X) higher than that obtained with the non-specific control peptide.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CD83 in Raji and Ramos cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CD83 in human tonsil tissue sections.

Flow Cytometry Analysis: 1 µg from a representative lot detected CD83 in one million Human peripheral blood mononuclear cells (PBMC).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

CD83 antigen (UniProt: Q01151; also known as hCD83, B-cell activation protein, Cell surface protein HB15) is encoded by the CD83 gene (Gene ID:9308) in human. CD83 is a single-pass type I membrane glycoprotein. It consists of an extracellular V-type Ig-like domain (aa 20-144), a transmembrane domain (aa 145-166), and a cytoplasmic tail (aa 167-205). It is thought to play a role in immune cells activation and immune tolerance. CD83 is expressed on a wide variety of cells, but primarily on the surface of activated immune cells, such as mature dendritic cells, T and B cells. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Its expression is upregulated upon dendritic cell maturation. Overexpression of CD83 is observed in certain autoimmune diseases while reduced expression or function of CD83 might contribute to immune evasion in cancers and chronic infections. Soluble CD83 has been studied for its potential therapeutic effects, such as prolonging graft survival in transplantation and ameliorating symptoms of autoimmune diseases. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Grosche, L. et al. (2020). Front. Immunol., 11; Article 721).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable