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Discovery® C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

product name

Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

agency

suitable for USP L1

feature

endcapped

manufacturer/tradename

Discovery®

extent of labeling

12% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

300 m2/g

surface coverage

3 μmol/m2

impurities

<10 ppm metals

matrix

silica gel, high purity, spherical particle platform

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

180 Å

operating pH range

2-8

application(s)

food and beverages

separation technique

reversed phase

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General description

Use Discovery® C18 for any method that specifies a C18. The exceptional peak shape, reproducibility, and stability make it the column of choice for all C18 methods from demanding to routine.

Features and Benefits

  • Excellent reproducibility
  • Exceptional peak shape for basic and acidic analytes
  • Stable, low-bleed LC-MS separations
  • Separation of peptides and small proteins
  • Lower hydrophobicity than many comparable C18 columns, providing faster analysis

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Product No.
Description
Pricing

G001085

HPLC Analysis of Angiotensins on Discovery® C18, application for HPLC

G000595

HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery® C18 after SPE using Discovery® DSC-18, application for SPE, application for HPLC

G001056

HPLC Analysis of Barbiturates in Serum on Discovery® C18 after SPE using Discovery® DSC-18Lt, application for SPE, application for HPLC

G001635

HPLC Analysis of 3-Methylpyrazole and 4-Methylpyrazole in Urine on Discovery® C18 after SPE using Discovery® DSC-SCX, application for SPE, application for HPLC

G003764

HPLC Analysis of Furosemide (Lasix) in Horse Serum on Discovery® C18 after SPE using Discovery® DSC-18, application for SPE, application for HPLC

G005684

HPLC Analysis of Caffeine Metabolites and Analogs on Discovery® C18, application for HPLC

guard cartridge

Product No.
Description
Pricing

related product

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
292187-06
292187-05
292187-04
292187-03
292187-02

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T R Shantha Kumar et al.
Journal of pharmaceutical and biomedical analysis, 38(1), 173-179 (2005-05-24)
A simple, precise and accurate isocratic reverse-phase liquid chromatography method with programmed wavelength detection has been validated to quantify DRF-4367 and Phenol red, simultaneously for application in rat in situ single pass intestinal perfusion study to assess intestinal permeability of
Abbas Khan et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(24), 2423-2429 (2011-07-26)
A novel isocratic reversed-phase high performance liquid-chromatography/ultraviolet detection method for simultaneous determination of cefdinir and cefixime in human plasma was developed and validated after optimization of various chromatographic conditions and other experimental parameters. Sample preparation based on a simple extraction
Himal Paudel Chhetri et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 22(5), 483-487 (2014-12-05)
This study presents the optimization of a simple HPLC-UV method for the determination of metformin in human plasma. Ion pair separation followed by UV detection was performed on deproteinized human plasma samples. The separation was carried out on a Discovery
Ramisetti Nageswara Rao et al.
Journal of separation science, 32(9), 1312-1322 (2009-04-29)
Modafinil, adrafinil and their related substances were synthesized and analyzed by RP-LC with ESI-MS/MS. The ionization mode, polarity, cone voltage, and chromatographic conditions were evaluated. The optimum LC-MS conditions to obtain fragment ions indispensable for identification of the structures were
Sheelendra Pratap Singh et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(27), 2845-2851 (2011-09-06)
Tamoxifen is the agent of choice for the treatment of estrogen receptor-positive breast cancer. Tamoxifen is a substrate of P-glycoprotein (P-gp) and microsomal cytochrome P450 (CYP) 3A, and biochanin A (BCA) is an inhibitor of P-gp and CYP3A. Hence, it
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Protocols

Extraction and Analysis of Lasix (Furosemide) in Horse Serum

Studies have suggested that there is a correlation between furosemide and improved racing performance, prompting the horse racing industry to monitor the use of this drug

Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns

Separations of Tetracycline Antibiotics by Reversed Phase HPLC, Using Discovery Columns

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