Documents
52973-U
HybridSPE® DPX Tip
weight 30 mg (bed), Tecan, tip volume 200 μL
Recommended Products
product line
HybridSPE®
composition
HybridSPE® sorbent
packaging
box of 96 × (tips)
technique(s)
solid phase extraction (SPE): suitable
tip volume
200 μL
weight
30 mg (bed)
matrix active group
Zirconia-based phase
application(s)
food and beverages
compatibility
Tecan
Related Categories
1 of 4
This Item | 52974-U | 52979-U | 52977-U |
|---|---|---|---|
| technique(s) solid phase extraction (SPE): suitable | technique(s) solid phase extraction (SPE): suitable | technique(s) solid phase extraction (SPE): suitable | technique(s) solid phase extraction (SPE): suitable |
| matrix active group Zirconia-based phase | matrix active group Zirconia-based phase | matrix active group Zirconia-based phase | matrix active group Zirconia-based phase |
| application(s) food and beverages | application(s) food and beverages | application(s) food and beverages | application(s) food and beverages |
| compatibility Tecan | compatibility Tecan | compatibility INTEGRA | compatibility Hamilton |
| product line HybridSPE® | product line HybridSPE® | product line HybridSPE® | product line HybridSPE® |
General description
In this simple technique, biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is extracted using the HybridSPE DPX tip which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids.
The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE® stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC/MS or LC/MS/MS analysis.
We offer an array of tips that are compatible with the most common liquid handling platforms and pipettors on the market: Tecan, Hamilton, INTEGRA, and a Universal format as well.
What size tips do I need?
There are two versions of the DPX tips available for the HybridSPE-PL. The standard HybridSPE DPX 50 mg tips are designed for use with biological sample volumes between 100-300 μL, while the HybridSPE DPX 30 mg tips are designed for use with biological sample volumes between 30-100 μL.
Guideline for 30 mg tips - plasma/serum sample: 30-100 μL; precipitating agent: 90-300 μL
Guideline for 50 mg tips - plasma/serum sample: 100-300 μL; precipitating agent: 300-900 μL
Features and Benefits
- Minimal elution solvent volumes
- Rapid extraction times (less than 3 minutes/sample)
- High extraction efficiencies
- Easy to perform extractions
- Lower costs
- Higher throughput
- Minimal training required
- Environmentally friendly
- Eliminates ion suppression effects often associated with phospholipids
- Tips offered to fit many formats: Tecan, Hamilton®, INTEGRA, and Universal liquid handling platforms and pipettors.
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(C)
Not applicable
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