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58220-U

Supelco

SUPELCOSIL LC-8 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Synonym(s):

LC-8 Reverse-Phase HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

SUPELCOSIL LC-8 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Quality Level

agency

suitable for USP L7

product line

SUPELCOSIL

feature

endcapped

packaging

1 ea of

extent of labeling

6.0% carbon loading

parameter

≤70 °C temp. range

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.2 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

C8 (octyl) phase

particle size

5 μm

pore size

120 Å

pH range

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

A phase less hydrophobic than C18. Provides less retention of both polar and non-polar compounds than C18. Use a mobile phase containing 5% less organic modifier for the C8 column than C18. Polar compounds are, relatively, more strongly retained on C8 than C18 columns.

Application


  • Chromatographic behaviour of ionic liquid cations in view of quantitative structure-retention relationship.: This study explores the chromatographic behavior of ionic liquid cations using quantitative structure-retention relationships (QSRR). The SUPELCOSIL LC-8 HPLC Column was utilized to analyze retention data for various ionic liquids, providing insights into their separation mechanisms and potential applications in analytical chemistry (Molíková et al., 2010).

  • Liquid chromatographic method for the determination of lidocaine and monoethylglycine xylidide in human serum containing various concentrations of bilirubin for the assessment of liver function.: The research presents a liquid chromatographic method using the SUPELCOSIL LC-8 HPLC Column to determine lidocaine and its metabolite in human serum. This method is particularly useful for assessing liver function by measuring drug metabolism (Piwowarska et al., 2004).

  • LC determination of morphine and morphine glucuronides in human plasma by coulometric and UV detection.: The research describes a method for the determination of morphine and its glucuronides in human plasma. Utilizing the SUPELCOSIL LC-8 HPLC Column, the study demonstrates the method′s sensitivity and applicability in pharmaceutical analysis (Ary et al., 2001).

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
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A HPLC-UV determination of clobazam and N-desmethylclobazam in human serum and urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions
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