Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

58295C40

Supelco

SUPELCOSIL™ LC-Si (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

SUPELCOSIL™ LC-Si HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm

material

stainless steel column

Quality Level

100

agency

suitable for USP L3

product line

SUPELCOSIL™

feature

endcapped: no

manufacturer/tradename

SUPELCOSIL™

packaging

1 ea of

parameter

≤70 °C temp. limit
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4 mm

surface area

170 m2/g

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

silica phase

particle size

5 μm

pore size

120 Å pore size

pH range

2-7.5

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent. Non-polar compounds will elute from the column first, while polar solutes show stronger interaction with the silanol groups on the silica surface. The polar selectivity of silica is very helpful for separating mycotoxins. Silica is frequently used in preparative chromatography due to its low operating cost and back pressure compared to reversed-phase columns. Silica is particularly popular among organic chemists as a low-pressure preparative tool. The unmodified silica in SUPELCOSIL LC-Si columns also separate positional isomers.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

guard cartridge

Product No.
Description
Pricing

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
291386-02
291386-01
291385-02
291385-01
290612-01

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mohammed Shahid Ali et al.
Journal of pharmaceutical and biomedical analysis, 43(1), 158-167 (2006-08-05)
A new HILIC method has been developed for the simultaneous determination of pseudoephedrine hydrochloride (PSH), diphenhydramine hydrochloride (DPH) and dextromethorphan hydrobromide (DXH) in cough-cold syrup. Mobile phase consists of methanol:water (containing 6.0 g of ammonium acetate and 10 mL of
R Lovlin et al.
Journal of chromatography. B, Biomedical applications, 679(1-2), 196-198 (1996-04-26)
The stereospecific HPLC assays reported for ketoprofen (KT) mainly utilize indirect approaches. These assays involve the formation of amide diastereomeric derivatives, which are then separated by chromatography. The advantages of indirect methods include versatility, good sensitivity and cost effectiveness; however
J J Agren et al.
Lipids, 37(6), 613-619 (2002-07-18)
Normal-phase HPLC resolution of sn-1,2(2,3)- and x-1,3-DAG generated by partial Grignard degradation from natural TAG was carried out with both (R)-(-) and (S)-(+)-1-(1-naphthyl)ethylurethane derivatives. The diastereomeric sn-1,2- and sn-2,3-DAG derivatives were resolved using two Supelcosil LC-Si (5 microm, 25 cm

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service