MilliporeSigma
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58934

Supelco

SUPELCOSIL Suplex pKb-100 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

eCl@ss:
32110501

Agency

suitable for USP L68

feature

endcapped: no

manufacturer/tradename

SUPELCOSIL

extent of labeling

12.5% carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g
170 m2/g

surface coverage

surface coverage 3.4 μmol/m2

matrix

silica gel, spherical particle platform

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

120 Å

pH range

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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This Item
58934C4058934C3058932
agency

suitable for USP L68

agency

suitable for USP L68

agency

suitable for USP L68

agency

suitable for USP L68

feature

endcapped: no

feature

endcapped: no

feature

endcapped: no

feature

endcapped: no

extent of labeling

12.5% carbon loading

extent of labeling

12.5% carbon loading

extent of labeling

12.5% carbon loading

extent of labeling

12.5% carbon loading

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

matrix

silica gel, spherical particle platform

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform, fully porous particle

matrix

silica gel, spherical particle platform

matrix active group

amide, alkyl phase

matrix active group

amide, alkyl phase

matrix active group

amide, alkyl phase

matrix active group

amide, alkyl phase

General description

Suplex pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL LC-ABZ columns. These specially deactivated columns differ in that Suplex pKb-100 is not endcapped, while SUPELCOSIL LC-ABZ is endcapped. The absence of the end-capping reagent results in better performance from Suplex pKb-100 for the strongest basic compounds, while LC-ABZ is preferred when the sample also contains acids and/or zwitterions.

Application

SUPELCOSIL Suplex pKb-100 HPLC Column was used in HPLC purification of prepurified fly ovaries. It was also used for HPLC analysis, done for the determination of retinoid.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Suplex is a trademark of Sigma-Aldrich Co. LLC

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