807573
TSKgel® Phenyl-5PW (10 µm) HPLC Columns
L x I.D. 7.5 cm × 7.5 mm, Phenyl phase
Synonym(s):
TSKgel® Hydrophobic Interaction Chromatography (HIC) Column
About This Item
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product name
TSKgel® Phenyl-5PW HPLC Column, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
material
PEEK
product line
TSKgel®
manufacturer/tradename
Tosoh Phenyl-5PW, 07573
parameter
≤4 M salt concentration
≤50% organic solvent
1.2 mL/min flow rate
10-50 °C temperature
20 bar max. pressure
technique(s)
HPLC: suitable
L × I.D.
7.5 cm × 7.5 mm
matrix
polymer particle platform
matrix active group
Phenyl phase
particle size
10 μm
pore size
1000 Å
operating pH
2-12
separation technique
hydrophobic interaction (HIC)
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General description
TSKgel BioAssist Phenyl columns contain the same particles as Phenyl-5PW columns, but are packed in PEEK column hardware rather than stainless steel. BioAsist Phenyl (Phenyl-5PW), TSKgel Ether-5PW and Butyl-NPR resin-based columns provide a range of hydrophobicities for chromatographic optimization. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing, such as BioAssist Phenyl. We recommend an Ether-5PW column for purifying very hydrophobic proteins. TSKgel BioAssist Phenyl and TSKgel Ether-5PW packings are based on TSKgel G5000PW resin - 10Â μm particles with 1000Â Ã… pores. TSKgel Butyl-NPR columns, which are less hydrophobic than Ether-5PW, is prepared from 2.5Â μm nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2Â M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.
Physical form
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Application
Guard column
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