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TSKgel® Protein C4-300 HPLC Column

phase C4 (butyl), L × I.D. 5 cm × 2 mm, 3 μm particle size

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stainless steel


suitable for USP L26

product line



Tosoh 22830

extent of labeling

3% Carbon, endcapped with trimethylsilane


no limitation salt concentration
0-100% organic solvent
1 mL/min standard flow rate
10-50 °C temperature
150 bar max. pressure


HPLC: suitable

L × I.D.

5 cm × 2 mm

surface area

100 m2/g


spherical silica

matrix active group

C4 (butyl) phase,

particle size

3 μm

operating pH


separation technique

reversed phase

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General description

TSKgel Protein C4-300 columns are the most recent addition to the extensive line of Reversed Phase columns from Tosoh Bioscience. The high efficiency columns are designed for the optimal recovery and resolution of proteins such as recombinant proteins, antibody fragments or PEGylated proteins. The 300 Å pore size of the TSKgel Protein C4-300 columns are ideal for the separation of proteins. A particle size of 3 μm and optimized ligand density and alkyl length result in better protein and peptide resolution compared to other leading RP-C4 HPLC phases. Latest surface modification techniques and endcapping of residual silanol groups reduce undesirable secondary interactions and peak tailing.

Physical form

Shipped in acetonitrile.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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