G003766
HPLC Analysis of Diazepam and Metabolites in Serum on Discovery® C18 after SPE using Discovery® DSC-8
application for SPE, application for HPLC
About This Item
technique(s)
HPLC: suitable
solid phase extraction (SPE): suitable
test parameters
sample preparation: SPE (Solid Phase Extraction)
sample/matrix: goat serum spiked with diazepam and metabolites at 0.5 μg/mL and diluted 1:1 in 25 mM ammonium formate, pH 7.1
SPE well plate: Discovery DSC-8 96-well SPE Plate, 100 mg/well (575627-U)
condition: 1 mL methanol followed by 1 mL DI water.
sample addition: 1 mL spiked goat serum
washing: 1 mL 25% methanol in 25 mM ammonium formate, pH 2.75
elution: 1 mL 60% methanol in 25 mM ammonium formate, pH 2.75
elution: Evaporate eluate with nitrogen purge (30 °C; ~15 min.). Reconstitute in 200 μL mobile phase.
column: Discovery C18, 5 cm x 4.6 mm I.D., 5 μm particles (504947)
mobile phase: [A] Methanol; [B]10 mM ammonium acetate, pH 4.5;(45:55, A:B)
flow rate: 1.5 mL/min
column temp.: 35 °C
detector: UV, 240 nm
injection: 25 μL
suitability
application for HPLC
application for SPE
application(s)
clinical
forensics and toxicology
Legal Information
Analytical column
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Sorry, we don't have COAs for this product available online at this time.
If you need assistance, please contact Customer Support.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service