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G005589

HPLC Analysis of Prednisolone (Secondary Pharma Standard) on Ascentis® C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)
mobile phase: [A] water; [B] methanol:acetonitrile (50:50, A:B)
gradient: 40% B for 14 min; to 80% B in 6 min; held at 80% B for 5 min
flow rate: 1 mL/min
column temp.: 40 °C
detector: UV, 254 nm
injection: 10 μL
sample: 125 μg/mL in water:acetonitrile (60:40)

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Prednisolone, pharmaceutical secondary standard; traceable to USP, PhEur and BP

Analytical column

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