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G005628

LC/MS Analysis of Vaccinium Anthocyanidins on Ascentis® Express C18 at Elevated Temperature and Flow

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] 0.1% formic acid in water; [B] 0.1% formic acid in 75:25 (v/v) acetonitrile:water; (78:22, A:B)
flow rate: 0.6 mL/min
pressure: 5440 psi (375 bar)
column temp.: 35 °C
detector: ESI(+), TOF
injection: 1 μL
sample: 20 μg/mL in 0.1% formic acid

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

Fruits of the genus Vaccinium are popular sources for anthocyanidin antioxidants. This genus includes the commonly known blueberry, huckleberry, and bilberry.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Delphinidin chloride
    Cyanidin chloride
    Pelargonidin chloride
    Peonidin chloride
    Malvidin chloride

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