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MilliporeSigma

G005662

HPLC Analysis of Aflatoxins on Ascentis® Express C18 with Fluorescence Detection

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)
mobile phase: [A] water; [B] acetonitrile; (75:25, A:B)
flow rate: 2.0 mL/min
pressure: 6381 psi (440 bar)
column temp.: 35 °C
detector: fluorescence, ex 360 nm, em 440 nm
injection: 50 μL
sample: 200 μL aflatoxin standard in acetonitrile; 0.5 μg/mL B2 and G2; 2 μg/mL B1 and G1; 800 μL delivatization solution: 70% water, 20% TFA and 10% acetic acid
heat for 9 min at 65 °C

suitability

application for HPLC

application(s)

food and beverages

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Aflatoxin G1
    Aflatoxin B1
    Aflatoxin G2
    Aflatoxin B2

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