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G006369

HPLC Analysis of PAHs in Canola Oil on SUPELCOSIL LC-PAH after SPE using Supelclean EZ-POP NP

application for SPE, application for HPLC

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technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: Canola oil spiked with PAHs at 10 ng/g
SPE tube/cartridge: Supelclean EZ-POP NP, 12 mL, pk of 20 (54341-U)
condition: 10 mL acetone, dry under 10-15" mercury for 10 minutes
sample addition: 0.5 mL weighed accurately onto cartridge
elution: 15 mL acetonitrile
eluate post-treatment: concentrate to final volume of 0.5mL at 40 °C under nitrogen
column: SUPELCOSIL LC-PAH 25 cm x 4.6 mm I.D., 5 μm particles (58229)
mobile phase: [A] water [B] acetonitrile
gradient: 40% B held for 5 min; to 100% B in 15 min; held at 100% B for 12 min
flow rate: 2.4 mL/min
pressure: 2790 psi at start (192 bar)
column temp.: 25 °C
detector: Fluorescence, programmed.

suitability

application for HPLC
application for SPE

application(s)

food and beverages

Analysis Note

PAHs are ubiquitous in the environment, resulting from both natural and manmade sources of combustion. They can contaminate food crops through environmental exposure to particulates, automobile exhaust, etc. In the case of edible oils, additional PAHs can be introduced during actual production processes such as seed drying [1]. In this application, EZ-POP NP SPE is used to extract PAHs from soybean oil. The 16 PAHs designated in many US EPA methods (less acenaphthylene) were analyzed by HPLC-FLD after extraction from a spiked sample of oil.

Other Notes

Detector: Naphthalene, Acenaphthene, Fluorene - 225nm/320nm; Phenanthrene, Anthracene - 250nm/368nm; Fluoranthene, Pyrene - 237nm/440nm; Benzo[a]anthracene, Chrysene - 265nm/380nm; Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[a]pyrene, Dibenzo[a,h]anthracene - 280nm/420nm; Benzo[g,h,i]perylene, Indeno[1,2,3-cd]pyrene, Benzo[b]chrysene - 300nm/466nm

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analyte

Description

    Naphthalene
    Acenaphthene
    Fluorene
    Phenanthrene
    Anthracene
    Fluoranthene
    Pyrene
    Benzo[a]anthracene
    Chrysene
    Benzo[b]fluoranthene
    Benzo[k]fluoranthene
    Benzo[a]pyrene
    Dibenzo[a,h]anthracene
    Benzo[g,h,i]perylene
    Indeno[1,2,3-cd]pyrene
    Benzo[b]chrysene (I.S.)
    See All (16)

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