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MilliporeSigma

LPLC Media & Accessories

LPLC packed columns with different media for specific chromatography applications.

Our extensive collection features a diverse range of high-quality media and accessories, meticulously designed to support LPLC separations across various applications. Whether its biomolecule purification, protein chromatography, or small molecule isolation, our LPLC products deliver exceptional performance and reliability.

Section Overview



Adsorbent Resins

Our selection of adsorbent resins includes alumina, carbon, hydrophobic dextran, Florisil®, Lipid Removal Agents, polymeric adsorbents, silicas, and bonded phase silicas. They provide high selectivity, excellent capacity, and robust stability with uniform particle size, optimized pore structure, high surface area, and chemical resistance. Explore our collection, including Supelpak™ resin, XAD™ resins, AmberLite™ resins, AmberChrom™ resins, AmberSep™ resins, DIAION™ resins, Carboxen™ resins, and SEPABEADS™ resins.

Affinity Resins

Our affinity chromatography media, which includes acrylic beads, agarose, cellulose, and Toyopearl® resins, have a high binding capacity, mechanical stability, and consistent performance, allowing for rapid and dependable biomolecule purification without shrinking or swelling at changing ionic strengths.

Gel Filtration Resins

Our gel filtration, or size exclusion, resins provide high resolution separation, a wide molecular weight range, and exceptional chemical stability. These resins separate biomolecules precisely and efficiently by size, resulting in great recovery and purity of proteins, nucleic acids, and other macromolecules. The resins show non-specific binding and compatibility with various buffers with reproducible results. Brands include Sephadex® resin, Avicel® resin, and Sepharose® resin.

Ion exchangers

We offer microporous (gel-type) and macroreticular (macroporous) ion exchangers. Gel-based ion exchange resins show high exchange capacity, microporosity (pore sizes up to 30 Angstroms), and strong resistance to acids, bases, oxidants, and temperature effects. Macroporous ion exchange resins have large pore diameters for improved ion exchange, high stability under chemical, thermal, and mechanical stress, and fast regeneration, making them ideal for dealing with high molecular weight pollutants while assuring long-term performance.

  • Strongly Acidic Cation Exchangers: The strongly acidic cation exchanger resins are highly stable to acids, bases, organic solvents, and reducing and oxidizing agents. Their ionization degree is comparable to very strong acids, and they are effective across the pH range of 0 to 14.
  • Strongly Basic Anion Exchangers: They are highly resistant to acids, bases, solvents, and reducing and oxidizing agents, allowing for enhanced regeneration and somewhat reduced silicic acid adsorption. Their exchange capability is pH-independent, with an ionization degree comparable to alkali hydroxides.
  • Weakly Acidic Cation Exchangers: Resistant to strong alkalis and solvents, they work well in pH ranges 6-14. They can be effectively regenerated with dilute mineral acids over time, as their ionization degree is comparable to that of acetic acid.
  • Weakly Basic Anion Exchangers: Effective over a pH range of 0 to 7, they offer excellent regeneration capability. They can be easily regenerated with NaOH, Na2CO3, or NH3, ensuring cost-effectiveness while minimizing theoretical capacity loss, making them perfect for cost-effective and environmentally friendly resin applications.
  • Mixed-bed Ion Exchangers: Our high-capacity mixed bed resins include strongly acidic cation exchange resin and strongly basic anion exchange resin with uniform particle size distribution. The mixed-bed resins have a convenient color indicator for easy monitoring of resin exhaustion. They also exhibit excellent regenerability, chemical compatibility, and durable performance, for an efficient and consistent removal of ion impurities from water.
  • Chelating resins: These are synthetic polymer beads containing functional groups that can make coordination covalent connections with metal ions. In a variety of industrial processes, they are employed to selectively remove and purify metal ions.
  • Nuclear Resin: Specialized ion exchange resins to purify and treat radioactive materials in nuclear power plants, research laboratories, and nuclear waste management facilities.

Related Resources

  • Article: Selection Guide for Polymeric Resins

    Polymeric resins offer research-scale separations and purification applications, including various adsorbent types.

  • Brochure: Resins Selection Guide

    LPLC is a versatile chromatographic technique that has evolved from using open columns and gravity to incorporating pumps and instrumentation. It offers a wide range of media, packed and empty columns, filters, injectors, valves, and tubing. The guide also covers Carboxen® Synthetic carbons, highly engineered derivatized resins with functions in adsorption and hydrophobic interaction chromatography.



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