Affinity Chromatography Resins

A laboratory setting showcasing the tools and chemicals used in affinity chromatography. In the foreground, three bottles labeled with hazard symbols indicate the presence of flammable and corrosive substances, essential for selective biochemical interactions and purification processes. In the background, a scientist in a lab coat is engaged with complex equipment, likely related to large-scale manufacturing and process development in affinity chromatography.

Affinity chromatography separates complex mixtures through highly selective biochemical interactions. This widely used purification technology efficiently separates a target molecule from impurities. Our range of affinity resins are designed for high throughput and cost-effective purification, from process development through large-scale manufacturing.

Protein A Affinity Resins
Affinity Resins for Plasma Purification
Affinity Resins for Protein Purification
Support for Qualification, Risk Assessment, and Process Optimization

Related Resources

Tool: mAbsizer™ Calculator
mAb Expression, Purification, and Formulation
PDF: Biochromatography Portfolio Guide
Bulk Resin, Membranes and Prepacked Columns
PDF: Chromatography Purification Selection Guide
Chromatography product selection guide.
Data Sheet: Eshmuno^® A Resin
Eshmuno^® A media is a rigid, high-capacity, acid and alkalineresistant Protein A affinity chromatography media for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies.
Data Sheet: Eshmuno^® P anti-A and anti-B Resins
High performance, acid and alkaline resistant affinity chromatography resins designed for the removal of anti-A and anti-B antibodies from plasma-derived immunoglobulin (Ig).
Data Sheet: Fractogel^®Chromatography Resin
Fractogel^® resins consist of synthetic methacrylate based polymeric beads providing excellent pressure stability resulting in high flow rates. Depending on your purification application, we can offer resin sized M-type beads with a particle size of 40–90 μm and small S-type beads with a particle size in the range of 20–40 μm.
Application Note: Using Ethylene Glycol Emprove^® Evolve to increase Eshmuno^®
Sodium hydroxide (NaOH) is a standard cleaning agent for protein A chromatography resins. Typically, a concentration of 0.1 mol/L NaOH is used. The inclusion of 20% ethylene glycol in a 0.1 mol/L NaOH cleaning solution increases the lifetime of Eshmuno^® A columns significantly.
Application Note: Detection and Quantification of Leached Protein A from Eshmuno^® A Resin Using Cygnus F400 Protein A ELISA Kit
Cygnus Technologies performed the initial feasibility testing of the Eshmuno^® A Protein A ligand in the Cygnus Protein A ELISA Kit (Cat# F400). Upon successful completion of the feasibility testing, Cygnus proceeded with quantification of the assay for use in in-process and drug substance samples using the Eshmuno^® A Protein A resin.
User Guide: MiniChrom^® Pre-Packed Columns
The MiniChrom^® columns are prepacked chromatography columns designed for laboratory-scale process development evaluations. When used for biomolecule separations, the results achieved with MiniChrom^® columns are typically similar to those obtained using traditional laboratory-scale columns from different vendors.

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Protein A Affinity Resins

Eshmuno^® A is a high-capacity resin for the purification of Fc-containing proteins, such as monoclonal antibodies (mAbs). This resin can be sanitized under acid and alkaline conditions.

Affinity Resins for Plasma Purification

Eshmuno^® P anti-A and anti-B resins effectively remove anti-A and anti-B isoagglutinin antibodies in plasma-derived Immunoglobulin (Ig) processes, which has been associated with increased patient risk for hemolysis.

Affinity Resins for Protein Purification

Fractogel^® Metal Chelate resin offers efficient and cost-effective separation of proteins and peptides, especially proteins with exposed histidine, cysteine, and tryptophan residues, including histidine-tagged proteins.

Support for qualification, risk assessment, and process optimization

Complementing our product portfolio, the Emprove^® Program provides three types of dossiers to support different stages of development and manufacturing operations such as qualification, risk assessment, and process optimization. The dossiers consolidate comprehensive product-specific testing data, quality statements, and regulatory information in a readily available format to simplify your compliance needs.