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Ion Exchange Chromatography Resins

Ion exchange (IEX) chromatography is a commonly used purification method that separates a target molecule from other impurities based on the net surface charge. Our portfolio of anion exchange (AEX) and cation exchange (CEX) resins, with our multi-modal chromatography resins, offers efficient purification solutions for mAbs, vaccines, plasmid DNA, plasma, and other modalities.

IEX chromatography products

Our industry-trusted Eshmuno® and Fractogel® resins offer purification efficiency and high yield resulting in improved process economics. The familiar column format can be easily scaled up from bench- to large-scale manufacturing.

Chromatography Eshmuno CMX

Eshmuno® IEX chromatography resins

These resins are ideal for efficient downstream purification and contain our proven tentacle ligand technology coupled with rigid base beads. They operate at fast flowrates and maintain high binding capacities, improving productivity by reducing time and manufacturing costs.

Eshmuno® CP-FT

Eshmuno® CMX mixed-mode resin provides high selectivity for mAbs, fusion proteins, and ADC purification, as well as separation of low molecular weight impurities and HCPs.

Eshmuno® CP-FT

Eshmuno® CP-FT resin efficiently removes mAb aggregates in flow-through mode, resulting in 10 times higher loading capacities as compared to traditional bind/elute CEX chromatography. This enables process intensification while reducing the overall cost for downstream mAb purification.

Eshmuno® CPS

Eshmuno® CPS resin offers high dynamic binding capacity and separation efficiency of recombinant protein feed streams at elevated salt concentrations. This enables direct loading of high-conductivity feed streams (conductivity ≥10 mS/cm), reducing the need for dilution.

Eshmuno® CPX

Eshmuno® CPX resin separates mAb monomers from aggregates and other impurities, even at high loading. It delivers outstanding productivity for high-resolution purification of process intermediates.

Eshmuno® HCX resin provides fast, efficient, direct capture of proteins, delivering outstanding performance in typical ion exchange and flow-through applications at higher salt levels.
Eshmuno® S is a strong CEX resin for direct capture of recombinant proteins and fast, efficient mAb purification following protein A capture.
Eshmuno® Q is a strong anion resin, offering outstanding impurity removal in typical anion exchange flow-through applications.

Fractogel® EMD IEX chromatography resins

All Fractogel® EMD resins provide high selectivity coupled with consistent pressure stability, enabling very high throughput and excellent yields. The resin’s high binding capacity, even at high flow rates, saves valuable time and improves process economics. Fractogel® resins are offered in different bead sizes with multiple functional groups to meet a range of purification needs:

Fractogel® strong anion exchangers

Fractogel® weak anion exchangers

Fractogel® strong cation exchangers

Fractogel® weak cation exchangers

Support for qualification, risk assessment, and process optimization

Complementing our product portfolio, the Emprove® Program provides three types of dossiers to support different stages of development and manufacturing operations such as qualification, risk assessment, and process optimization. The dossiers consolidate comprehensive product-specific testing data, quality statements, and regulatory information in a readily available format to simplify your compliance needs.

Related Product Resources

mAb Expression, Purification, and Formulation

mAbsizer™ Calculator

Product Selection

Guide: Biochromatography Portfolio
Guide: Chromatography Purification Selection

Product Specifications

Data Sheet: Eshmuno® CMX Resin
Data Sheet: Eshmuno® CP‑FT Resin
Data Sheet: Data Sheet: Eshmuno® CPS
Data Sheet: Eshmuno® HCX Media
Data Sheet: Eshmuno® S Resin for Superior Downstream mAB Purification
Data Sheet: Eshmuno® Q Resin for Efficient AEX Chromatography

Product Operations

Application Note: ADC Separation Using a Mixed Mode Cation Exchange Chromatography Step in a mAb Process
Application Note: Intensified Polishing Using Single-pass Tangential Flow Filtration (SPTFF) with Anion Exchange Chromatography
Application Note: Eshmuno® CMX Application Note on Glycovariant Separation Including for COVID-19
Article: Cation Exchange Frontal Chromatography for the Removal of Monoclonal Antibody Aggregates
Article: Viral Clearance in Antibody Purification Using Tentacle Ion Exchangers Article: Bioprocessing Technology Trends of RNA-based Therapeutics and Vaccines
Article: RNA-Based Therapeutics and Vaccines
Article: Design Space and Robustness Analysis of Batch and Counter-current Frontal Chromatography Processes for the Removal of Antibody Aggregates
Article: Process Intensification by Frontal Chromatography
Poster: Eshmuno® CMX resin - A Novel Mixed Mode Cation Exchange Resin for the Purification of Glycoproteins
Animation: Separation of Low or Too High DAR Antibody Drug Conjugate Species
Poster:Comparison of Three Tentacular Strong Cation Exchange Resins that Have the Same Base Bead
Poster:High-Capacity Capture of a Recombinant Growth Factor Directly from Refold Solution Using Salt Tolerant Cation Exchange Chromatography
Tech Note: Development Strategy for a Cation Exchange (CEX) Chromatography Step in a Monoclonal Antibody (mAb) Process
User Guide: MiniChrom® Pre-Packed Columns

Products

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Product Number
Product Description
Pricing
1.16883
Fractogel® EMD DEAE (M), weak anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
1.25072
MiniChrom Column Fractogel® SO3 (M), 5 ml, column I.D. × L 8 mm × 100 mm
1.10316
Fractogel® EMD TMAE Hicap (M), strong anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
1.16881
Fractogel® EMD TMAE (M), strong anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
1.25070
MiniChrom Column Fractogel® TMAE Hicap (M), 5 ml, column I.D. × L 8 mm × 100 mm
1.16882
Fractogel® EMD SO₃⁻ (M), strong cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
1.25069
MiniChrom Column Fractogel® TMAE (M), 5 ml, column I.D. 8 mm, bed L 100 mm
1.20083
Eshmuno® CPX
1.20079
Eshmuno® Q
1.16884
Fractogel® EMD DMAE (M), weak anion exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
1.25136
RoboColumn Fractogel® TMAE (M), 0.6 ml, column I.D. × L 5 mm × 30 mm
1.14894
Fractogel® EMD SE Hicap (M), strong cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
1.25082
MiniChrom Column Fractogel® COO (M), 5 ml, column I.D. × L 8 mm × 100 mm
1.16887
Fractogel® EMD TMAE (S), strong anion exchanger, suspension in 20% ethanol and 150 mM NaCl (20-40 µm)
1.20093
Eshmuno® CP-FT
1.25062
MiniChrom Column Fractogel® COO (M), 1 ml, column I.D. × L 8 mm × 20 mm
1.25055
MiniChrom Column Fractogel® TMAE Hicap (M), 1 ml, column I.D. × L 8 mm × 20 mm
1.16886
Fractogel® EMD COO⁻ (M), weak cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)
1.20078
Eshmuno® S
1.25080
MiniChrom Column Fractogel® DMAE (M), 5 ml, column I.D. × L 8 mm × 100 mm
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Highlights

Webinar: Exploring Enhanced Selectivity of Innovative Ion Exchange Resin in ADC Polishing
Webinar: Exploring Enhanced Selectivity of Innovative Ion Exchange Resin in ADC Polishing

New technologies enabling efficient DAR species separation with the possibility to reduce manufacturing costs & simplify process optimization 

Webinar: A Novel CEX Resin for the Removal of mAb Aggregate via Flow-through Frontal Chromatography
Webinar: A Novel CEX Resin for the Removal of mAb Aggregate via Flow-through Frontal Chromatography

CEX flow-through frontal chromatography for the removal of mAb aggregates as well as the conditions that influence the removal of these aggregates

Video: Vaccine tentacle technology

Eshmuno® CMX mixed-mode chromatography resin for the purification of vaccines

Video: Explore Efficiency: Eshmuno® CP‑FT Resin

Explore the significant cost savings in mAb downstream purification by utilizing Eshmuno® CP-FT Resin

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