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MilliporeSigma

1.16882

Millipore

Fractogel® EMD SO3 ⁻ (M) Resin

Synonym(s):

Fractogel® EMD SO₃⁻ (M)

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About This Item

UNSPSC Code:
41115711

Quality Level

ligand

(Sulfoisobutyl)

description

strong cation exchanger, suspension in 20% ethanol and 150 mM NaCl (40-90 µm)

sterility

sterile (Caustic Stable)

product line

Fractogel®

form

resin

specific analyte(s)

MAbs

parameter

8 bar max. pressure

matrix active group

methacrylate

mean particle size

40-90 μm

capacity

130 mg binding capacity (lysozyme/ml of resin)

transition temp

flash point 35 °C (Does not sustain combustion.)

density

1.43 g/cm3 at 20 °C

bulk density

1000 kg/m3

application(s)

recombinant protein
vaccine development

separation technique

cation exchange
strong cation exchange

storage temp.

2-30°C

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General description

Strong cation exchange Fractogel® resin featuring the tentacle technology, for purification of basic and neutral proteins (e.g. antibodies) and peptides

Features and Benefits

Fractogel® EMD SO3- (M) enables:

  • Increased binding strength at low pH
  • High aggregate removal efficiency combined with high dynamic binding capacity
  • Lower elution volumes for the highest purity levels
  • Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution


Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel® EMD SO3- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:
  • the buffer system
  • pH value of the buffer which determines the surface charge of the protein
  • the concentration of the counter ions
  • the charge density on the support (protein binding capacity)

Packaging

  • 1.16882.0100: Fractogel® EMD SO3- (M) Resin 100ml
  • 1.16882.0010: Fractogel® EMD SO3- (M) Resin 10ml
  • 1.16880.0500: Fractogel® EMD SO3- (M) Resin 500ml
  • 1.16882.5000: Fractogel® EMD SO3- (M) Resin 5L

Plastic bottle

Analysis Note

Appearance: Milky turbid suspension,free from impurities(foreign particles)
Microscopic evaluation: Uniform spherical particles,no agglomerates, no fines
Extractable matter (water): ≤ 0.05 %
Cerium: ≤ 1 µg/g
Pressure drop(column: ID=1.6 cm, L=10 cm at 5 ml/min): ≤ 1.0 bar
Particle size (d10): 37 - 45 µm
Particle size (d50): 48 - 60 µm
Particle size (d90): 63 - 77 µm
Colony forming units (TAMC + TYMC): ≤ 100 CFU/ml
Endotoxins: ≤ 1.00 EU/ml
Protein binding capacity (lysozyme): 110 - 150 mg/ml
Functional test (b:a): ≤ 0.4
Functional test (c:d): ≤ 0.45
Functional test: Separation chymotrypsinogen A, cytochrom C and lysozyme

Legal Information

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

95.0 °F

flash_point_c

35 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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