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  • GATA4 as a novel regulator involved in the development of the neural crest and craniofacial skeleton via Barx1.

GATA4 as a novel regulator involved in the development of the neural crest and craniofacial skeleton via Barx1.

Cell death and differentiation (2018-03-11)
Shuyu Guo, Yuxin Zhang, Tingting Zhou, Dongyue Wang, Yajuan Weng, Qi Chen, Junqing Ma, Yi-Ping Li, Lin Wang
ABSTRACT

The role of GATA-binding protein 4 (GATA4) in neural crest cells (NCCs) is poorly defined. Here we showed that mouse NCCs lacking GATA4 exhibited developmental defects in craniofacial bone, teeth, and heart. The defects likely occurred due to decreased cell proliferation at the developmental stage. The in vitro results were consistent with the mouse model. The isobaric tags for relative and absolute quantitation assay revealed that BARX1 is one of the differentially expressed proteins after GATA4 knockdown in NCCs. On the basis of the results of dual-luciferase, electro-mobility shift, and chromatin immunoprecipitation assays, Barx1 expression is directly regulated by GATA4 in NCCs. In zebrafish, gata4 knockdown affects the development of NCCs derivatives. However, the phenotype in zebrafish could be partly rescued by co-injection of gata4 morpholino oligomers and barx1 mRNA. This study identified new downstream targets of GATA4 in NCCs and uncovered additional evidence of the complex regulatory functions of GATA4 in NCC development.

MATERIALS
Product Number
Brand
Product Description

Roche
DIG RNA Labeling Kit (SP6/T7), sufficient for 2 x 10 labeling reactions, kit of 1 (12 components), suitable for hybridization, suitable for Southern blotting
Sigma-Aldrich
Alcian Blue 8GX, powder
Sigma-Aldrich
Anti-SOX9 Antibody, from rabbit, purified by affinity chromatography
Roche
In Situ Cell Death Detection Kit, TMR red, sufficient for ≤50 tests