MilliporeSigma
All Photos(4)

Documents

A5268

Sigma-Aldrich

Alcian Blue 8GX

powder

Sign Into View Organizational & Contract Pricing

Synonym(s):
Alcian Blue, Ingrain Blue 1
Empirical Formula (Hill Notation):
C56H68Cl4CuN16S4
CAS Number:
Molecular Weight:
1298.86
Colour Index Number:
74240
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, 45-65%

color

blue

solubility

H2O: 1 mg/mL

εmax

425 at 615-630 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C

InChI

1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4

InChI key

CKLBXIYTBHXJEH-UHFFFAOYSA-J

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A3157C8679A9186
vibrant-m

A5268

Alcian Blue 8GX

vibrant-m

A3157

Alcian Blue 8GX

vibrant-m

C8679

Chicago Sky Blue 6B

vibrant-m

A9186

Alcian Blue 8GX

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

εmax

425 at 615-630 nm in water

εmax

195-400 at 617-630 nm

εmax

-

εmax

-

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

-

form

powder

form

powder

form

powder

form

powder

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

General description

Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.

Application

  • Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.
  • It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models.
  • It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species.

Features and Benefits

Desired staining patterns can be obtained by manipulating pH and salt concentrations.

Principle

The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 2

1 of 2

DIRECT DYE (C.I. 31855) AldrichCPR

S469858

DIRECT DYE (C.I. 31855)

Nuclear Fast Red

Sigma-Aldrich

N8002

Nuclear Fast Red

Histotechnology: A Self-instructional Text (3rd ed.)
Freida L. Carson & Christa Hladik
Histotechnology: A Self Instructional Text, 4th Edition (2009)
In vivo visualization of Bonghan ducts inside blood vessels of mice by using an Alcian blue staining method
Yoo, et al.
Indian Journal of Experimental Biology, 46 (2008)
Replacement of the medial tibial plateau by a metallic implant in a goat model.
Custers RJ, et al.
Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, 28, 429-435 (2010)
Selective COX-2 inhibition prevents proinflammatory cytokine-induced cartilage damage.
Mastbergen SC, et al.
Rheumatology (Oxford, England), 41, 801-808 (2002)
Exposure of human cartilage tissue to low concentrations of blood for a short period of time leads to prolonged cartilage damage: an in vitro study.
Jansen NW, et al.
Arthritis and Rheumatism, 56, 199-207 (2007)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service