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A5533

Sigma-Aldrich

Alizarin Red S

certified by the Biological Stain Commission

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Synonym(s):
3,4-Dihydroxy-9,10-dioxo-2-anthracenesulfonic acid sodium salt, Alizarin Carmine, Alizarin Red, water soluble, Alizarin sodium monosulfonate, Alizarin sulfonate sodium, Alizarinsulfonic acid sodium salt, Sodium alizarinsulfonate
Empirical Formula (Hill Notation):
C14H7NaO7S
CAS Number:
Molecular Weight:
342.26
Colour Index Number:
58005
Beilstein/REAXYS Number:
4117090
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

grade

certified by the Biological Stain Commission

Quality Level

form

powder

concentration

0.015 g/L in 0.1 M NaOH

technique(s)

titration: suitable

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Oc1c(O)c(cc2C(=O)c3ccccc3C(=O)c12)S([O-])(=O)=O

InChI

1S/C14H8O7S.Na/c15-11-6-3-1-2-4-7(6)12(16)10-8(11)5-9(22(19,20)21)13(17)14(10)18;/h1-5,17-18H,(H,19,20,21);/q;+1/p-1

InChI key

HFVAFDPGUJEFBQ-UHFFFAOYSA-M

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1 of 4

This Item
114502M8256199303
Alizarin Red S certified by the Biological Stain Commission

A5533

Alizarin Red S

Methyl Red sodium salt ACS reagent, Dye content 95 %

114502

Methyl Red sodium salt

Methyl Red sodium salt Crystalline

M8256

Methyl Red sodium salt

Resazurin sodium salt certified by the Biological Stain Commission

199303

Resazurin sodium salt

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

titration: suitable

technique(s)

-

concentration

0.015 g/L in 0.1 M NaOH

concentration

-

concentration

-

concentration

-

solubility

H2O: 1 mg/mL

solubility

water: 0.1%

solubility

soluble, clear, orange

solubility

1 M NH4OH: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

powder

form

powder

form

powder

Application

  • Alizarin Red S has been used for checking the calcium deposits in osteogenic culture.
  • It has been used for the quantitative analyses of calcification in aortic explants.
  • It has been used for mineral staining in osteoblast cultures.

Biochem/physiol Actions

Alizarin Red S is used for the staining of cartilage and bone. It reacts with calcium, thereby helping in the diagnosis of calcium deposits. It is also used to study carbohydrate–boronic acid interactions.

Suitability

Certified for use in Dahl′s method for calcium

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Customers Also Viewed

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On the history and mechanism of alizarin and alizarin red S stains for calcium.
Puchtler H, et al.
The Journal of Histochemistry and Cytochemistry, 17, 110-124 (1969)
Phenol red inhibits chondrogenic differentiation and affects osteogenic differentiation of human mesenchymal stem cells in vitro.
Lysdahl H, et al.
Stem Cell Reviews, 9, 132-132 (2013)
Alizarin Red S. as a general optical reporter for studying the binding of boronic acids with carbohydrates.
Chemical Communications (Cambridge, England), 17, 1608-1609 (2001)
RGD peptides immobilized on a mechanically deformable surface promote osteoblast differentiation.
Cavalcanti-Adam EA, et al.
Journal of Bone and Mineral Research, 17, 2130-2130 (2002)
Pathologic calcification of adult vascular smooth muscle cells differs on their crest or mesodermal embryonic origin.
Leroux-Berger M, et al.
Journal of Bone and Mineral Research, 26, 1543-1543 (2011)

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