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  • Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells.

Release of potassium, lipids, and proteins from nonionic detergent treated chicken red blood cells.

Journal of cellular physiology (1994-05-01)
M Kellermayer, A Ludány, A Miseta, T Kŏszegi, G Berta, P Bogner, C F Hazlewood, I L Cameron, D N Wheatley
ABSTRACT

The plasma membrane of erythrocytes, as of other cells, is thought to act as the barrier responsible for maintaining intracellular gradients of most ions and small molecular species between the cell and its environment. Controlled application of the nonionic detergent Brij 58 effectively opened the erythrocyte plasma membrane, as judged by electron microscopy and lipid mobilization, but the cytoplasm maintained much of its integrity for about 30 min. Release of K+ correlated well with release of protein into the surrounding medium. The results demonstrate that permeabilization of the erythrocyte plasma membrane does not result in an instantaneous equilibration of small ions, such as K+, between the cell and its environment. A comparison was made between erythrocytes treated with Brij 58 and Triton X-100. The lipid and protein solubilizing actions of Triton X-100 were not as easily separable in time as those of Brij 58. The results of treatment of the erythrocytes with different types of nonionic detergents suggest that the membranolytic and cytoplasmic protein destabilizing actions of nonionic detergents correspond with their hydrophilic-lipophilic balance numbers (HLB values).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Brij® 58, average Mn ~1124