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  • Laminariales Host Does Impact Lipid Temperature Trajectories of the Fungal Endophyte Paradendryphiella salina (Sutherland.).

Laminariales Host Does Impact Lipid Temperature Trajectories of the Fungal Endophyte Paradendryphiella salina (Sutherland.).

Marine drugs (2020-07-28)
Marine Vallet, Tarik Meziane, Najet Thiney, Soizic Prado, Cédric Hubas
ABSTRACT

Kelps are colonized by a wide range of microbial symbionts. Among them, endophytic fungi remain poorly studied, but recent studies evidenced yet their high diversity and their central role in algal defense against various pathogens. Thus, studying the metabolic expressions of kelp endophytes under different conditions is important to have a better understanding of their impacts on host performance. In this context, fatty acid composition is essential to a given algae fitness and of interest to food web studies either to measure its nutritional quality or to infer about its contribution to consumers diets. In the present study, Paradendryphiella salina, a fungal endophyte was isolated from Saccharina latissima (L.) and Laminaria digitata (Hudson.) and its fatty acid composition was assessed at increasing salinity and temperature conditions. Results showed that fungal composition in terms of fatty acids displayed algal-dependent trajectories in response to temperature increase. This highlights that C18 unsaturated fatty acids are key components in the host-dependant acclimation of P. salina to salinity and temperature changes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Methyl tricosanoate, ≥99.0% (GC)
Supelco
Boron trifluoride-methanol solution, ~10% (~1.3 M), for GC derivatization, LiChropur
Sigma-Aldrich
Tricosanoic acid, ≥99% (capillary GC)
Supelco
Bacterial Acid Methyl Ester (BAME) Mix, solution (10 mg/mL total concentration in methyl caproate), analytical standard