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  • Development and characterization of swine primary respiratory epithelial cells and their susceptibility to infection by four influenza virus types.

Development and characterization of swine primary respiratory epithelial cells and their susceptibility to infection by four influenza virus types.

Virology (2019-01-08)
Chithra C Sreenivasan, Milton Thomas, Linto Antony, Tristen Wormstadt, Michael B Hildreth, Dan Wang, Ben Hause, David H Francis, Feng Li, Radhey S Kaushik
ABSTRACT

Influenza viruses are a group of respiratory pathogens that have evolved into four different types: A, B, C, and D. A common feature is that all four types are capable of replicating and transmitting among pigs. Here, we describe the development of isogenous cell culture system from the swine respiratory tract to study influenza viruses. Phenotypic characterization of swine primary nasal turbinate, trachea and lung cells revealed high expression of cytokeratin and demonstrated tissue site dependent expression of tight junction proteins. Furthermore, lectin binding assay on these cells demonstrated higher levels of Sia2-6Gal than Sia2-3Gal receptors and supported the replication of influenza A, B, C, and D viruses to appreciable levels at both 33 and 37 °C, but replication competence was dependent on virus type or temperature used. Overall, these swine primary respiratory cells showed epithelial phenotype, which is suitable for studying the comparative biology and pathobiology of influenza viruses.

MATERIALS
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Product Description

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