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M9269

Sigma-Aldrich

IgG1, Kappa from murine myeloma

clone MOPC 21, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Mouse IgG1-κ
MDL number:

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

MOPC 21, monoclonal

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
M9035I3784M1421
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

MOPC 21, monoclonal

clone

MOPC 31C, monoclonal

clone

DX, monoclonal

clone

TEPC 15, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Specificity

Specificity is determined by mouse monoclonal isotyping strips. The purified immunoglobulin preparation is non-reactive with anti mouse IgA, IgM, IgG2a, IgG2b or IgG3

Application

IgG1, κ from murine myeloma has been used in:
  • the in situ hybridization experiments with bulbar conjunctiva sections
  • immunohistochemistry of cattle and artery tissues
IgG1, κ from murine myeloma has been used:
  • in immunofluorescence of leukocytes at a concentration of 50 μg/ml
  • as a standard in enzyme linked immunosorbent assay (ELISA)
  • as exchange antibody in centrifugal gel filtration
IgG1, Kappa from murine myeloma has been used in flow cytometric analysis and immunoprecipitation.

Biochem/physiol Actions

IgG antibody subtype is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
IgG1 has a molecular weight of 146 kDa and is an abundantly expressed IgG subclass. IgG1 deficiencies contribute to overall decrease in total IgG levels resulting in hypogammaglobulinemia. IgG1 promotes bacterial phagocytosis and mediates vaccine-induced protection from infection. The ratio of IgG2a to IgG1 is crucial for the immune response against Leishmania tropica infection. It has high binding affinity towards the Fcγ receptors (FcγR). High levels of IgG1-κ antibodies is associated with the pathology of glomerulonephritis.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0, containing 0.02% sodium azide

Storage and Stability

Store at −20 °C. The product may be stored frozen in working aliquots at −20 °C. Repeated freezing and thawing is not recommended.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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The relative contribution of mast cell subsets to conjunctival TH2-like cytokines.
Anderson DF, et al.
Investigative Ophthalmology & Visual Science, 42(5), 995-1001 (2001)
M Dellian et al.
British journal of cancer, 82(9), 1513-1518 (2000-05-02)
Molecular charge is one of the main determinants of transvascular transport. There are, however, no data available on the effect of molecular charge on microvascular permeability of macromolecules in solid tumours. To this end, we measured tumour microvascular permeability to
Elin Borgen et al.
Breast cancer research : BCR, 20(1), 120-120 (2018-10-17)
The presence of disseminated tumor cells (DTCs) in bone marrow (BM) is an independent prognostic factor in early breast cancer but does not uniformly predict outcome. Tumor cells can persist in a quiescent state over time, but clinical studies of
B Steppich et al.
American journal of physiology. Cell physiology, 279(3), C578-C586 (2000-08-16)
Strenuous, anaerobic exercise leads to an increase of leukocytes that are mobilized from the marginal pool. We have analyzed in human peripheral blood the effect of exercise on the number of CD14(+)CD16(+) monocytes as determined by two-color immunofluorescence and flow
G G de Sá Oliveira et al.
Clinical and experimental immunology, 105(2), 313-320 (1996-08-01)
The specificities of a panel of erythrocyte-reactive MoAbs derived from NZB mice with autoimmune haemolytic anaemia (AIHA) were determined by immunoprecipitation and immunoblotting. Of the eight antibodies, two (IgG1 MoAb 105-2H and IgG2a MoAb 34-3C) immunoprecipitated a 105-kD component identified

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