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MABC002

Sigma-Aldrich

Mouse IgG1 Negative Control, clone Ci4

Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

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eCl@ss:
32160702
NACRES:
NA.42

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

Ci4, monoclonal

manufacturer/tradename

Chemicon®

concentration

0.5 mg/mL

technique(s)

flow cytometry: suitable
immunofluorescence: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

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This Item
CBL610AP124CAP107
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

F(ab′)2 fragment of affinity isolated antibody

antibody form

purified immunoglobulin

clone

Ci4, monoclonal

clone

1E2.2, monoclonal

clone

polyclonal

clone

polyclonal

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

concentration

0.5 mg/mL

concentration

1 mg/mL

concentration

-

concentration

-

technique(s)

flow cytometry: suitable, immunofluorescence: suitable

technique(s)

flow cytometry: suitable

technique(s)

immunofluorescence: suitable

technique(s)

ELISA: suitable, immunoprecipitation (IP): suitable, western blot: suitable

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Reacts with chicken immunoglobulin. No reaction with human cell surface or plasma components has been observed. However some binding to Fc receptors may occur.

Immunogen

Chicken immunoglobulin

Application

Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.
Mouse IgG1 is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry, and immunoperoxidase staining on frozen and paraffin tissue sections. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used.
Research Category
Secondary & Control Antibodies

Epitope Tags & General Use
Research Sub Category
Isotype Control Antibodies

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in phosphate buffered saline, pH 7.4, containing 0.2% bovine Serum albumin and 0.1% sodium azide.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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