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Sigma-Aldrich

Normal Rabbit IgG

Normal Rabbit IgG Polyclonal Antibody control validated for use in Immunoprecipitation & Western Blotting.

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

polyclonal

manufacturer/tradename

Upstate®

concentration

1 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

target post-translational modification

unmodified

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This Item
12-342AP132-M12-348
Normal Rabbit IgG Normal Rabbit IgG Polyclonal Antibody control validated for use in Immunoprecipitation & Western Blotting.

12-370

Normal Rabbit IgG

Sigma-Aldrich

AP132-M

Goat Anti-Rabbit IgG Antibody

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

peroxidase conjugate

conjugate

unconjugated

conjugate

peroxidase conjugate

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunofluorescence: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

concentration

1 mg/mL

concentration

-

concentration

2 mg/mL

concentration

1 mg/mL

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Application

Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Quality

Routinely evaluated by IP/WB as a non-specific IgG control.

Immunoprecipitation/Western Blot Assay:
2 µg of this lot was used as a non-specific IgG control in an immunoprecipitation / western blot assay.

Physical form

Purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide before the addition of glycerol to 30%. Liquid at –20°C.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product centrifuge the original vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Regulation of the PMP22 gene through an intronic enhancer.
Jones, EA; Lopez-Anido, C; Srinivasan, R; Krueger, C; Chang, LW; Nagarajan, R; Svaren, J
The Journal of Neuroscience null
Chromatin immunoprecipitation assay for tissue-specific genes using early-stage mouse embryos.
Ok Hyun Cho,Jaime A Rivera-Perez,Anthony N Imbalzano
Journal of Visualized Experiments null
Genome-wide profiling of DNA-binding proteins using barcode-based multiplex Solexa sequencing.
Sunil Kumar Raghav,Bart Deplancke
Methods in Molecular Biology null
Kinetics of facultative heterochromatin and polycomb group protein association with the herpes simplex viral genome during establishment of latent infection.
Cliffe, AR; Coen, DM; Knipe, DM
mBio null
Transcription-dependent silencing of inducible convergent transgenes in transgenic mice.
Calero-Nieto, FJ; Bert, AG; Cockerill, PN
Epigenetics & Chromatin null

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