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I5404

Sigma-Aldrich

IgG2, Kappa from human myeloma plasma

purified immunoglobulin, >95% (SDS-PAGE)

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Synonym(s):
Human IgG2-κ
MDL number:
NACRES:
NA.46

biological source

human

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

Assay

>95% (SDS-PAGE)

shipped in

dry ice

storage temp.

−20°C

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This Item
I5654I5154I5029
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

assay

>95% (SDS-PAGE)

assay

>90% (microfluidic capillary gel electrophoresis)

assay

>95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections.
Human myeloma IgG2, κ is purified from human plasma by fractionation, ion-exchange and affinity chromatography procedures.

Application

The purified IgG2, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG2, κa was used to characterize the trisulfide modifications in human and mouse antibodies and in flow cytometry.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0.

Analysis Note

Identity is determined by immunoelectrophoresis and indirect ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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