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I5154

Sigma-Aldrich

IgG1, Kappa from human myeloma plasma

purified immunoglobulin, >95% (SDS-PAGE)

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Synonym(s):
Human IgG1-κ
MDL number:
NACRES:
NA.46

biological source

human

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

Assay

>95% (SDS-PAGE)

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

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This Item
I5029I5404M9035
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

assay

>95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥95% (gel filtration, HPLC)

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG1 is the most abundant of the IgG subclass
Human myeloma IgG1, κ is purified from human plasma by fractionation, ion-exchange, and affinity chromatography procedures.

Application

The purified IgG1, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent or coating protein in a variety of immunoassays including ELISA, dot-blot immunobinding, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays. Human myeloma IgG1, κ was injected into mice (500 μg) to assess the T cell and B cell responses and also used for flow cytometry in CHO cells.

Physical form

Solution in 0.02 M Tris buffered saline, pH 8.0.

Analysis Note

Identity is determined by immunoelectrophoresis and indirect ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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