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Group I p21-activated kinases in leukemia cell adhesion to fibronectin.

Cell adhesion & migration (2021-01-20)
Kateřina Kuželová, Adam Obr, Pavla Röselová, Dana Grebeňová, Petra Otevřelová, Barbora Brodská, Aleš Holoubek
ABSTRACT

P21-activated kinases (PAK) regulate processes associated with cytoskeleton dynamics. PAK expression in leukemia cells was measured on protein and mRNA levels. In functional assays, we analyzed the effect of PAK inhibitors IPA-3 and FRAX597 on cell adhesivity and viability. PAK2 was dominant in cell lines, whereas primary cells also expressed comparable amount of PAK1 transcription isoforms: PAK1-full and PAK1Δ15. PAK1Δ15 and PAK2 levels correlated with surface density of integrins β1 and αVβ3. PAK1-full, but not PAK2, was present in membrane protrusions. IPA-3, which prevents PAK activation, induced cell contraction in semi-adherent HEL cells only. FRAX597, which inhibits PAK kinase activity, increased cell-surface contact area in all leukemia cells. Both inhibitors reduced the stability of cell attachment and induced cell death.

MATERIALS
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Product Description

Sigma-Aldrich
Monoclonal Anti-β-Actin antibody produced in mouse, clone AC-15, ascites fluid
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250 mL
Estimated to ship on March 24, 2025
$518.00
Sigma-Aldrich
Histopaque®-1077 Hybri-Max, liquid, sterile-filtered, BioReagent, suitable for hybridoma
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250 mL
Estimated to ship on March 24, 2025
$518.00
Sigma-Aldrich
IPA-3, ≥95% (HPLC), powder
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SKUPack SizeAvailabilityPriceQuantity
250 mL
Estimated to ship on March 24, 2025
$518.00