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  • Composition and structure of cell wall ulvans recovered from Ulva spp. along the Swedish west coast.

Composition and structure of cell wall ulvans recovered from Ulva spp. along the Swedish west coast.

Carbohydrate polymers (2020-02-16)
Niklas Wahlström, Filip Nylander, Eric Malmhäll-Bah, Karin Sjövold, Ulrica Edlund, Gunnar Westman, Eva Albers
ABSTRACT

The cell wall polysaccharide ulvan was isolated from two species of the seaweed Ulva collected along the Swedish west coast. Acidic extraction was benchmarked against hot water extraction with enzymatic purification and against commercial ulvan. Extracted ulvan contained 11-18 % g/g of ash, some protein (up to 1.3 % g N/g) but minimal colored impurities. The ulvans had high molecular weights (660,000-760,000 g/mol) and were composed of 77-79 % g/g carbohydrates, mainly rhamnose, xylose, glucose, glucuronic acid, and iduronic acid. The extraction protocol and the ulvan source strongly impact the molecular weight and the chemical composition. Acidic extraction caused almost complete desulfation of the isolated ulvan while the other method preserved a significant degree of SO3 substituents. Elemental analysis of ash remaining after thermal degradation showed presence of common mineral elements such as Na, Ca, Mg, Al, and K, but none of the heavy metals Pb, Hg, or As.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sulfate Assay Kit, sufficient for 200 tests (turbidimetric)