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  • Immunoglobulins on the surface of differently charged polymer nanoparticles.

Immunoglobulins on the surface of differently charged polymer nanoparticles.

Biointerphases (2020-06-04)
Domenik Prozeller, Christine Rosenauer, Svenja Morsbach, Katharina Landfester
ABSTRACT

The overall success of nanocarriers in biomedical applications depends on their interaction with different proteins in blood. Immunoglobulins as a major protein class of the blood proteome may considerably influence the identity of the nanocarriers in blood. However, there is a lack of knowledge about the specific details of the interaction mechanism between different immunoglobulins and nanocarriers. Therefore, the authors have investigated the interaction of different immunoglobulin classes-namely, immunoglobulin G, A, and M-with different polystyrene model nanoparticles. The authors report that immunoglobulin interaction with nanoparticles strongly depends on the immunoglobulin class and surface charge of the nanoparticles. Furthermore, upon adsorption on the nanoparticles' surfaces, aggregation processes and denaturation of immunoglobulins were observed. This highlights the importance of nanocarriers' design in order to prevent unfavorable denaturation and adsorption processes of immunoglobulins on nanoparticle surfaces.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
IgA from human serum, reagent grade, ≥95% (HPLC), buffered aqueous solution
Sigma-Aldrich
IgG from human serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Sigma-Aldrich
IgM from human serum, reagent grade, ~95% (HPLC), buffered aqueous solution