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  • Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA.

Production of the HBc Protein from Different HBV Genotypes in E. coli. Use of Reassociated HBc VLPs for Packaging of ss- and dsRNA.

Microorganisms (2021-02-13)
Ivars Petrovskis, Ilva Lieknina, Andris Dislers, Juris Jansons, Janis Bogans, Inara Akopjana, Jelena Zakova, Irina Sominskaya
ABSTRACT

The core proteins (HBc) of the hepatitis B virus (HBV) genotypes A, B, C, D, E, F, and G were cloned and expressed in Escherichia coli (E. coli), and HBc-formed virus-like particles (VLPs) were purified with ammonium sulfate precipitation, gel filtration, and ion exchange chromatography (IEX). The best VLP yield was found for the HBc of the HBV genotypes D and G. For the HBc of the HBV genotypes D, F, and G, the possibility of dissociation and reassociation maintaining the native HBc structure was demonstrated. Single-stranded (ss) and double-stranded (ds) ribonucleic acid (RNA) was successfully packed into HBc VLPs for the HBV genotypes D and G.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
Dialysis tubing cellulose membrane, avg. flat width 25 mm (1.0 in.)
Sigma-Aldrich
TRI Reagent®, For processing tissues, cells cultured in monolayer or cell pellets