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  • SARS-CoV-2 infection of human iPSC-derived cardiac cells reflects cytopathic features in hearts of patients with COVID-19.

SARS-CoV-2 infection of human iPSC-derived cardiac cells reflects cytopathic features in hearts of patients with COVID-19.

Science translational medicine (2021-03-17)
Juan A Perez-Bermejo, Serah Kang, Sarah J Rockwood, Camille R Simoneau, David A Joy, Ana C Silva, Gokul N Ramadoss, Will R Flanigan, Parinaz Fozouni, Huihui Li, Pei-Yi Chen, Ken Nakamura, Jeffrey D Whitman, Paul J Hanson, Bruce M McManus, Melanie Ott, Bruce R Conklin, Todd C McDevitt
ABSTRACT

Although coronavirus disease 2019 (COVID-19) causes cardiac dysfunction in up to 25% of patients, its pathogenesis remains unclear. Exposure of human induced pluripotent stem cell (iPSC)-derived heart cells to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) revealed productive infection and robust transcriptomic and morphological signatures of damage, particularly in cardiomyocytes. Transcriptomic disruption of structural genes corroborates adverse morphologic features, which included a distinct pattern of myofibrillar fragmentation and nuclear disruption. Human autopsy specimens from patients with COVID-19 reflected similar alterations, particularly sarcomeric fragmentation. These notable cytopathic features in cardiomyocytes provide insights into SARS-CoV-2-induced cardiac damage, offer a platform for discovery of potential therapeutics, and raise concerns about the long-term consequences of COVID-19 in asymptomatic and severe cases.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bovine Serum Albumin, cold ethanol fraction, pH 5.2, ≥96%
Sigma-Aldrich
DMEM Complete Medium, with 2mM L-Glut, Sodium Pyruvate, and 10% FBS, DMEM Complete Medium contains DMEM high glucose medium with 2mM L-glut, sodium pyruvate, and 10% U.S. origin FBS. DMEM Complete Medium is a convenient sterile solution for the culture of primary mammalian cells including mouse and chicken cells, and a variety of normal and transformed cells.
Sigma-Aldrich
Triton X-100, for molecular biology