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  • Transient co-expression for fast and high-yield production of antibodies with human-like N-glycans in plants.

Transient co-expression for fast and high-yield production of antibodies with human-like N-glycans in plants.

Plant biotechnology journal (2009-05-09)
Louis-P Vézina, Loïc Faye, Patrice Lerouge, Marc-André D'Aoust, Estelle Marquet-Blouin, Carole Burel, Pierre-Olivier Lavoie, Muriel Bardor, Véronique Gomord
ABSTRACT

Plant-based transient expression is potentially the most rapid and cost-efficient system for the production of recombinant pharmaceutical proteins, but safety concerns associated with plant-specific N-glycosylation have hampered its adoption as a commercial production system. In this article, we describe an approach based on the simultaneous transient co-expression of an antibody, a suppressor of silencing and a chimaeric human beta1,4-galactosyltransferase targeted for optimal activity to the early secretory pathway in agroinfiltrated Nicotiana benthamiana leaves. This strategy allows fast and high-yield production of antibodies with human-like N-glycans and, more generally, provides solutions to many critical problems posed by the large-scale production of therapeutic and vaccinal proteins, specifically yield, volume and quality.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Mouse IgG1 (heavy chain specific) antibody produced in goat, affinity isolated antibody, lyophilized powder
Sigma-Aldrich
IgG1, Kappa from murine myeloma, clone MOPC 21, purified immunoglobulin, buffered aqueous solution