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  • Development and validation of a confirmatory HPLC method for simultaneous determination of Sudan dyes in animal tissues and eggs.

Development and validation of a confirmatory HPLC method for simultaneous determination of Sudan dyes in animal tissues and eggs.

Journal of chromatographic science (2010-01-09)
Yin-Liang Wu, Cun Li, Xi Xia, Yong-Jun Liu, Jian-Zhong Shen
ABSTRACT

A simple and sensitive high-performance liquid chromatography (HPLC) analytical method for the simultaneous determination of six Sudan dyes (Sudan Red G, Sudan I, Sudan II, Sudan III, Sudan Red 7B, Sudan IV) in animal tissues and eggs was developed. Samples were extracted with acetonitrile followed by a cleanup using a C(18) solid-phase extraction column. Chromatographic separation was achieved on a Zorbax SB-C(18) column under gradient conditions. The analytes were detected at 510 nm by HPLC with diode array detection. Specificity, decision limit (CCalpha), detection capacity (CCbeta), accuracy, and precision were determined during validation process. Recoveries for six Sudan dyes from three animal tissues and eggs were 77.2-98.0% with excellent relative standard deviations. CCalpha and CCbeta were in the range of 7.7-9.0 microg/kg and 9.1-10.3 microg/kg, respectively. The limits of quantitations were between 12.8 microg/kg and 15.0 microg/kg.

MATERIALS
Product Number
Brand
Product Description

Supelco
Sudan Red 7B, analytical standard
Supelco
Sudan III, analytical standard
Sigma-Aldrich
Sudan III, Technical grade
Sigma-Aldrich
Sudan III, BioXtra, certified by the Biological Stain Commission
Sigma-Aldrich
Sudan Red 7B, for microscopy (Bot., Hist.)
Sigma-Aldrich
Sudan Red 7B
Supelco
Sudan II, analytical standard
Supelco
Sudan IV, analytical standard
Sigma-Aldrich
Sudan red G, reagent for Ph. Eur.
Sigma-Aldrich
Sudan I, Dye content ≥95 %
Sigma-Aldrich
Sudan II, Dye content 90 %
Sigma-Aldrich
Sudan IV, Certified Biological Stain Commission,Dye content ≥80 %
Sigma-Aldrich
Sudan IV, BioXtra, certified by the Biological Stain Commission
Supelco
Sudan I, analytical standard