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  • Multivariate image analysis-thin layer chromatography (MIA-TLC) for simultaneous determination of co-eluting components.

Multivariate image analysis-thin layer chromatography (MIA-TLC) for simultaneous determination of co-eluting components.

The Analyst (2010-04-28)
Bahram Hemmateenejad, Nabiollah Mobaraki, Fatemeh Shakerizadeh-Shirazi, Ramin Miri
ABSTRACT

This paper addresses the solution of peak overlapping, as a fundamental problem in TLC, by multivariate analysis of the images recorded by a digital camera. We report the results of our study on the application of multivariate image analysis (MIA) for simultaneous determination of several species on thin layer chromatography (TLC) sheet for the first time. An imaging system, composed of a dark cabinet, a digital camera and a multivariate image analysis program, was prepared for recording the images of TLC plates after development of a multi-component solution. The written program was able to produce 2- and 3-dimensional chromatograms of the solutions, which were subsequently used as inputs of partial least squares, as an efficient multivariate calibration method. The ability of the proposed MIA-TLC method for simultaneous determination of the co-eluting components was validated by analysis of ternary synthetic mixtures of indicators of highly overlapped chromatograms (i.e., methyl yellow, bromocresol green and creseol red) and a real mixture of nifedipine and its photo-degradation product. By application of different strategies like principal component analysis and variable selection, models were obtained that could estimate the concentration of indicators in the external prediction set with relative errors of lower than 10% and in most cases lower than 5%.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bromocresol Green, JIS special grade
Sigma-Aldrich
Bromocresol Green, ACS reagent, Dye content 95 %
Sigma-Aldrich
Bromocresol Green solution, pH 3.8-5.4
Sigma-Aldrich
Cresol red, JIS special grade
Sigma-Aldrich
Cresol Red sodium salt, indicator grade
Sigma-Aldrich
Bromocresol Green Sultone Form, for microscopy (Bot., Hist., Vit.), indicator (pH 3.8-5.4)
Sigma-Aldrich
Cresol red, indicator grade, Dye content 95 %