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  • [Selection of the optimal method of extraction and determination of dye Sudan I-IV and Para Red by high-performance liquid chromatography].

[Selection of the optimal method of extraction and determination of dye Sudan I-IV and Para Red by high-performance liquid chromatography].

Voprosy pitaniia (2010-06-22)
V V Bessononv, O I Perederiaev, E P Khromchenkova, Iu V Vedishcheva
ABSTRACT

Since 2004 was important the problem of determination of non-food dyes as Sudan I-IV and Para Red. Therefore it was necessary to develop reliable methodologies or adapt existing analytical methods for monitoring the presence of such dyes in food. For this purpose, we have previously proposed a method for high-performance liquid chromatography with diode-array and mass detection. However this method showed the urgent need to clarify this important phase of the study as the sample preparation. In the present work was studied the possibility of solving this problem such solvents as ethanol, chloroform, acetone, conducted the selection of the optimum ratio of extraction agent-raw. The authors have also refined the metrological characteristics of the method.

MATERIALS
Product Number
Brand
Product Description

Supelco
Sudan III, analytical standard
Sigma-Aldrich
Sudan III, BioXtra, certified by the Biological Stain Commission
Sigma-Aldrich
Sudan III, Technical grade
Sigma-Aldrich
Sudan I, Dye content ≥95 %
Sigma-Aldrich
Sudan IV, Certified Biological Stain Commission,Dye content ≥80 %
Supelco
Sudan I, analytical standard
Sigma-Aldrich
Sudan IV, BioXtra, certified by the Biological Stain Commission
Supelco
Sudan II, analytical standard
Supelco
Sudan IV, analytical standard
Sigma-Aldrich
Sudan II, Dye content 90 %