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  • Molecularly imprinted solid-phase extraction combined with ultrasound-assisted dispersive liquid-liquid microextraction for the determination of four Sudan dyes in sausage samples.

Molecularly imprinted solid-phase extraction combined with ultrasound-assisted dispersive liquid-liquid microextraction for the determination of four Sudan dyes in sausage samples.

The Analyst (2011-05-11)
Hongyuan Yan, Jindong Qiao, Hui Wang, Gengliang Yang, Kyung Ho Row
ABSTRACT

A simple and highly selective molecularly imprinted solid-phase extraction (MISPE) combined with ultrasound-assisted dispersive liquid-liquid microextraction (DLLME) was developed for the determination of four Sudan dye (I, II, III, and IV) residues in sausage products. The novel molecularly imprinted microspheres (MIMs) synthesized by aqueous suspension polymerization using phenylamine-naphthol as the dummy template show high affinity to the four Sudan dyes and were applied as selective sorbents of MISPE-DLLME to overcome the drawbacks of template leakage in quantitative analysis. Good linearity was obtained in a range of 0.005-2.0 μg g(-1) and the average recoveries of the four Sudan dyes at three spiked levels ranged from 86.3 to 107.5%. The MISPE-DLLME-HPLC protocol significantly improved the purification and enrichment of the analytes and eliminated the template leakage of the conventional MISPE on quantitative analysis.

MATERIALS
Product Number
Brand
Product Description

Supelco
Sudan III, analytical standard
Sigma-Aldrich
Sudan III, BioXtra, certified by the Biological Stain Commission
Sigma-Aldrich
Sudan III, Technical grade
Sigma-Aldrich
Sudan I, Dye content ≥95 %
Sigma-Aldrich
Sudan IV, Certified Biological Stain Commission,Dye content ≥80 %
Supelco
Sudan I, analytical standard
Sigma-Aldrich
Sudan IV, BioXtra, certified by the Biological Stain Commission
Supelco
Sudan II, analytical standard
Supelco
Sudan IV, analytical standard
Sigma-Aldrich
Sudan II, Dye content 90 %