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  • Biosynthesis and properties of medium-chain-length polyhydroxyalkanoates with enriched content of the dominant monomer.

Biosynthesis and properties of medium-chain-length polyhydroxyalkanoates with enriched content of the dominant monomer.

Biomacromolecules (2012-08-09)
Xuan Jiang, Zhiyong Sun, Robert H Marchessault, Juliana A Ramsay, Bruce A Ramsay
ABSTRACT

When grown in a nonanoic acid-limited chemostat at a dilution rate of 0.25 h(-1), Pseudomonas putida KT2440 produced poly(3-hydroxynonanoate-co-3-hydroxyheptanoate) containing 68 mol % 3-hydroxynonanoate (C9) and 32 mol % 3-hydroxyheptanoate (C7). Under the same conditions, but in the presence of acrylic acid, a fatty acid β-oxidation inhibitor, the C9 monomer content increased to 88 mol %. Cofeeding glucose (3.9 g L(-1)) and nonanoic acid (2.9 ± 0.1 g L(-1)) in continuous culture with 0.2 g L(-1) of acrylic acid in the feed, further increased the C9 content to 95 mol %. A yield of PHA from nonanoic acid of 0.93 mol mol(-1) was attained. PHA with a 3-hydroxyoctanoate (C8) content of 98 mol % was produced with the same cofeeding methodology from octanoic acid. As the dominant monomer content increased, the melting point of the poly(3-hydroxynonanoate) copolymers increased from 46 to 63 °C and that of the poly(3-hydroxyoctanoate) copolymers from 54 to 62 °C. All copolymer compositions resulted in elongation to break values of about 1300%, but tensile strength at break and Young's modulus both increased with increasing amounts of the dominant monomer.

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Nonanoic acid, analytical standard
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Octanoic acid, analytical standard
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Nonanoic acid, 96%
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Octanoic acid, ≥98%, FG
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Nonanoic acid, ≥96%, FG
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Octanoic acid, natural, ≥98%, FG
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Octanoic acid, ≥98%
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Nonanoic acid, ≥97%
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Octanoic acid, ≥99%
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Octanoic acid, ≥96.0%
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Sodium octanoate, ≥99% (capillary GC)
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Caprylic Acid (Octanoic Acid), Pharmaceutical Secondary Standard; Certified Reference Material
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