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  • Environmental and seasonal influences on red raspberry flavour volatiles and identification of quantitative trait loci (QTL) and candidate genes.

Environmental and seasonal influences on red raspberry flavour volatiles and identification of quantitative trait loci (QTL) and candidate genes.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik (2012-08-15)
Alistair Paterson, Angzzas Kassim, Susan McCallum, Mary Woodhead, Kay Smith, Dzeti Zait, Julie Graham
ABSTRACT

Raspberry volatiles are important for perceptions of sensory quality, mould resistance and some have nutraceutical activities. Twelve raspberry character volatiles were quantified, 11 of them in fruit from two seasons, from plants from the Glen Moy × Latham mapping population growing in both open field and under cover (polytunnels). Effects of season and environment were examined for their impact on the content of α-ionone, α-ionol, β-ionone, β-damascenone, linalool, geraniol, benzyl alcohol, (Z)-3-hexenol, acetoin, acetic and hexanoic acids, whilst raspberry ketone was measured in one season. A significant variation was observed in fruit volatiles in all progeny between seasons and method of cultivation. Quantitative trait loci were determined and mapped to six of the seven linkage groups, as were candidate genes in the volatiles pathways.

MATERIALS
Product Number
Brand
Product Description

Supelco
Acetoin, analytical standard
Supelco
Linalool, analytical standard
Sigma-Aldrich
Acetoin, natural, ≥95%, FG
Supelco
Butylated Hydroxytoluene, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Geraniol, analytical standard
Sigma-Aldrich
Butylated hydroxytoluene, ≥99%, FCC, FG
Sigma-Aldrich
Acetoin, May exist as crystalline dimer
Sigma-Aldrich
Acetoin, primarily dimer, ≥95%, FG
Sigma-Aldrich
Geraniol, ≥97%, FCC, FG
Sigma-Aldrich
Linalool, ≥97%, FCC, FG
Sigma-Aldrich
Geraniol, 98%
Sigma-Aldrich
Geraniol, natural, ≥97%, FG
Sigma-Aldrich
Linalool, 97%
Sigma-Aldrich
Benzyl alcohol, ≥99%, FCC, FG
Sigma-Aldrich
Benzyl alcohol, anhydrous, 99.8%
Sigma-Aldrich
Benzyl alcohol, natural, ≥98%, FG
Sigma-Aldrich
cis-3,7-Dimethyl-2,6-octadien-1-ol, ≥97%, FCC, FG
Sigma-Aldrich
L-Linalool, natural, ≥95%, FG
Sigma-Aldrich
2,6-Di-tert-butyl-4-methylphenol, tested according to Ph. Eur.
Sigma-Aldrich
(−)-Linalool, ≥95.0% (sum of enantiomers, GC)
Sigma-Aldrich
2,6-Di-tert-butyl-4-methylphenol, purum, ≥99.0% (GC)
Supelco
Benzyl alcohol, analytical standard
Supelco
(−)-Linalool, analytical standard
Supelco
3,5-Di-tert-butyl-4-hydroxytoluene, analytical standard
Sigma-Aldrich
2,6-Di-tert-butyl-4-methylphenol, ≥99.0% (GC), powder
Butylhydroxytoluene, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
cis-3,7-Dimethyl-2,6-octadien-1-ol, 97%
Supelco
Benzyl alcohol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Benzyl alcohol, SAJ special grade, ≥99.0%
Sigma-Aldrich
2,6-Di-tert-butyl-4-methylphenol, SAJ first grade, ≥99.0%