- DNA duplex stabilization in crowded polyanion solutions.
DNA duplex stabilization in crowded polyanion solutions.
Chemical communications (Cambridge, England) (2013-01-11)
Imran Khimji, Jeehae Shin, Juewen Liu
PMID23302897
ABSTRACT
The melting temperature of duplex DNA is much higher in polyanions than in non-ionic polymers with similar ionic strength, suggesting an additional electrostatic contribution on top of the excluded volume effect.
MATERIALS
Product Number
Brand
Product Description
Supelco
Poly(acrylic acid sodium salt), analytical standard, suitable for gel permeation chromatography (GPC), 1,250
Supelco
Poly(acrylic acid sodium salt), analytical standard, suitable for gel permeation chromatography (GPC), 16,000
Sigma-Aldrich
Poly(acrylic acid) solution, average Mw ~2,000, 50 wt. % in H2O, electronic grade