Skip to Content
MilliporeSigma
  • Comprehensive analysis of alterations in the miRNome in response to photodynamic treatment.

Comprehensive analysis of alterations in the miRNome in response to photodynamic treatment.

Journal of photochemistry and photobiology. B, Biology (2013-03-08)
Doris Bach, Julia Fuereder, Michael Karbiener, Marcel Scheideler, Anna Lena Ress, Daniel Neureiter, Ralf Kemmerling, Otto Dietze, Markus Wiederstein, Frieder Berr, Kristjan Plaetzer, Tobias Kiesslich, Martin Pichler
ABSTRACT

Photodynamic therapy (PDT) is a local tumour treatment accepted for a number of indications. PDT operates via the cellular stress response through the production of reactive oxygen species and subsequent cellular damage, resulting in cell death. Although PDT-induced signalling and cytotoxicity mechanisms have been investigated, the effect of PDT on microRNA (miRNA) expression is largely unknown. Therefore, we conducted a comprehensive microarray-based analysis of the miRNome of human epidermoid carcinoma cells (A431) following in vitro photodynamic treatment using polyvinylpyrrolidone hypericin (PVPH) as a photosensitiser and nearly homogeneous apoptosis-inducing conditions. Using microarray analysis we found eight miRNAs to be significantly differentially expressed 5h post treatment compared with the baseline levels and three miRNAs with more than 2-fold differential expression that could be detected in 1 or 2 biological replicates. The verification of these results by quantitative RT-PCR including a detailed time-course revealed an up to 15-fold transient over-expression of miR-634, miR-1246, miR-1290 and miR-487b compared with the basal level. For these miRNAs, in silico mRNA target prediction yielded numerous target transcripts involved in the regulation of cell stress, apoptosis, cell adherence and proliferation. This study provides the first comprehensive miRNome analysis after PDT treatment and may help to develop novel miRNA-based therapeutic approaches to further increase the efficiency of PDT.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Povidone, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Kollidon® 25
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Povidone, European Pharmacopoeia (EP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Supelco
Povidone, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, for molecular biology, nucleic acid hybridization tested, mol wt 360,000
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, mol wt (number average molecular weight Mn 360kDa)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, powder, BioXtra, suitable for mouse embryo cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, average mol wt 10,000
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, suitable for plant cell culture, average mol wt 10,000
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, average mol wt 40,000
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, K 30
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, powder, average Mw ~55,000
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, powder, average Mw ~29,000
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, K 90
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, average Mw ~1,300,000 by LS
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone, K 25, tested according to Ph. Eur.
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00
Sigma-Aldrich
Polyvinylpyrrolidone solution, K 60, 45% in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
20 g
In Stock
Details...
$146.00