A 5-day method for determination of soluble silicon concentrations in nonliquid fertilizer materials using a sodium carbonate-ammonium nitrate extractant followed by visible spectroscopy with heteropoly blue analysis: single-laboratory validation.

A 5-day method for determination of soluble silicon concentrations in nonliquid fertilizer materials using a sodium carbonate-ammonium nitrate extractant followed by visible spectroscopy with heteropoly blue analysis: single-laboratory validation.

Journal of AOAC International (2013-06-19)

Dennis Sebastian, Hugh Rodrigues, Charles Kinsey, Gaspar Korndörfer, Hamilton Pereira, Guilherme Buck, Lawrence Datnoff, Stephen Miranda, Mary Provance-Bowley

PMID23767347

ABSTRACT

A 5-day method for determining the soluble silicon (Si) concentrations in nonliquid fertilizer products was developed using a sodium carbonate (Na2CO3)-ammonium nitrate (NH4NO3) extractant followed by visible spectroscopy with heteropoly blue analysis at 660 nm. The 5-Day Na2CO3-NH4NO3 Soluble Si Extraction Method can be applied to quantify the plant-available Si in solid fertilizer products at levels ranging from 0.2 to 8.4% Si with an LOD of 0.06%, and LOQ of 0.20%. This Si extraction method for fertilizers correlates well with plant uptake of Si (r2 = 0.96 for a range of solid fertilizers) and is applicable to solid Si fertilizer products including blended products and beneficial substances. Fertilizer materials can be processed as received using commercially available laboratory chemicals and materials at ambient laboratory temperatures. The single-laboratory validation of the 5-Day Na2CO3-NH4NO3 Soluble Si Extraction Method has been approved by The Association of American Plant Food Control Officials for testing nonliquid Si fertilizer products.

MATERIALS

Product Number

Brand

Product Description

71363

Supelco

Sodium carbonate, reference material for titrimetry, certified by BAM, >99.5%

71345

Sigma-Aldrich

Sodium carbonate, BioUltra, anhydrous, ≥99.5% (calc. on dry substance, T)

451614

Sigma-Aldrich

Sodium carbonate, anhydrous, powder, 99.999% trace metals basis

577782

Sigma-Aldrich

Sodium carbonate decahydrate, 99.999% trace metals basis

S7795

Sigma-Aldrich

Sodium carbonate, BioXtra, ≥99.0%

223484

Sigma-Aldrich

Sodium carbonate, ACS reagent (primary standard), anhydrous, 99.95-100.05% dry basis

223530

Sigma-Aldrich

Sodium carbonate, powder, ≥99.5%, ACS reagent

13418

Sigma-Aldrich

Sodium carbonate, puriss., meets analytical specification of Ph. Eur., BP, NF, FCC, E500, anhydrous, 99.5-100.5% (calc. to the dried substance)

900504

Sigma-Aldrich

Sodium carbonate, anhydrous, free-flowing, Redi-Dri^™, ACS reagent (primary standard), 99.95-100.05% dry basis

13568

Sigma-Aldrich

Sodium carbonate monohydrate, puriss., meets analytical specification of Ph. Eur., BP, NF, FCC, E500, 99.5-100.5% (ex dried subst.)

S2127

Sigma-Aldrich

Sodium carbonate, ReagentPlus^®, ≥99.5%

S4132

Sigma-Aldrich

Sodium carbonate monohydrate, ReagentPlus^®, ≥99.5%

222321

Sigma-Aldrich

Sodium carbonate, ACS reagent, anhydrous, ≥99.5%, powder or granules

230952

Sigma-Aldrich

Sodium carbonate monohydrate, ACS reagent, ≥99.5%

900503

Sigma-Aldrich

Sodium carbonate, anhydrous, powder or granules, free-flowing, Redi-Dri^™, ACS reagent, ≥99.5%

71360

Sigma-Aldrich

Sodium carbonate decahydrate, puriss. p.a., ≥99.0% (T)

900505

Sigma-Aldrich

Sodium carbonate, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99.5%

56169

Supelco

Sodium carbonate concentrate, 0.1 M Na₂CO₃ in water, eluent concentrate for IC

01-4830

Sigma-Aldrich

Ammonium nitrate, JIS special grade, ≥99.0%

256064

Sigma-Aldrich

Ammonium nitrate, 99.999% trace metals basis

490741

Sigma-Aldrich

Sodium carbonate-¹²C, 99.9 atom % ¹²C

215619

Sigma-Aldrich

Silicon, powder, −325 mesh, 99% trace metals basis

343250

Sigma-Aldrich

Silicon, pieces, 99.95% trace metals basis

A7455

Sigma-Aldrich

Ammonium nitrate, BioXtra, ≥99.5%

A3795

Sigma-Aldrich

Ammonium nitrate, suitable for plant cell culture

647772

Sigma-Aldrich

Silicon, wafer (single side polished), <111>, P-type, contains boron as dopant, diam. × thickness 3 in. × 0.5 mm

647675

Sigma-Aldrich

Silicon, wafer (single side polished), <100>, P-type, contains boron as dopant, diam. × thickness 2 in. × 0.5 mm

647543

Sigma-Aldrich

Silicon, wafer (single side polished), <111>, N-type, contains no dopant, diam. × thickness 3 in. × 0.5 mm

647799

Sigma-Aldrich

Silicon, wafer (single side polished), <111>, N-type, contains phosphorus as dopant, diam. × thickness 2 in. × 0.5 mm

647802

Sigma-Aldrich

Silicon, wafer (single side polished), <100>, N-type, contains phosphorus as dopant, diam. × thickness 3 in. × 0.5 mm