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  • Sweet preference modified by early experience in mice and the related molecular modulations on the peripheral pathway.

Sweet preference modified by early experience in mice and the related molecular modulations on the peripheral pathway.

Journal of molecular neuroscience : MN (2013-04-23)
Wei-Li Li, Meng-Ling Chen, Si-Si Liu, Guo-Liang Li, Tian-Yuan Gu, Pei Liang, Yu-Mei Qin, Yue-Hua Zhan, Ying Quan, Gen-Hua Zhang
ABSTRACT

The sweet taste is of immense interest to scientists and has been intensively studied during the last two decades. However, the sweet preference modification and the related mechanisms are still unclear. In this study, we try to establish a mice model with manipulated sweet taste preference and explore the involved possible molecular mechanisms. The animals were exposed to acesulfame-K via maternal milk during lactation and the sweet preference tests were carried out when they grew to adulthood. Our results showed that the preference thresholds for sweet taste were increased in adults by early acesulfame-K exposure and the preference ratios for sweet tastants at low or preferred concentrations were decreased. Moreover, by means of qRT-PCR and Western blot, we observed the increased expression of leptin receptor Ob-Rb and downregulation of Gα-gustducin protein in the soft palate. Thereby, the sweet taste sensitivity may be modified by early sweetener experience during lactation. Along the peripheral sweet sensory pathway, the sweet regulator receptors Ob-Rb, CB1 and components of sweet transduction signal Gα-gustducin and T1R2 in both the soft palate and tongue may be cooperatively involved in the plastic development of sweet taste.

MATERIALS
Product Number
Brand
Product Description

Supelco
Acesulfame K, for food analysis, ≥99.0%
Acesulfame potassium, European Pharmacopoeia (EP) Reference Standard
Supelco
Acesulfame K, Pharmaceutical Secondary Standard; Certified Reference Material