Skip to Content
MilliporeSigma
  • Determination of aflatoxins B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil using immunoaffinity column cleanup, postcolumn derivatization, and liquid chromatography with fluorescence detection: first action 2013.05.

Determination of aflatoxins B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil using immunoaffinity column cleanup, postcolumn derivatization, and liquid chromatography with fluorescence detection: first action 2013.05.

Journal of AOAC International (2013-11-29)
Lei Bao, Chengzhu Liang, Mary W Trucksess, Yanli Xu, Ning Lv, Zhenxing Wu, Ping Jing, Fred S Fry
ABSTRACT

A collaborative study of a method for determination of aflatoxins (AFs) B1, B2, G1, and G2 in olive oil, peanut oil, and sesame oil using immunoaffinity column cleanup, postcolumn derivatization, and LC with fluorescence detection, previously published in J. AOAC Int. 95, 1689-1700 (2012), was approved as First Action 2013.05 on March 29, 2013 by the Method-Centric Committee for Aflatoxins in Edible Oils. The method uses methanol for extraction followed by filtration. The extract is applied to an immunoaffinity column with antibodies specific for AFs, which are then eluted from the column with a methanol solution. Determination and quantification occur using RP-LC with fluorescence detection after postcolumn derivatization. The average recovery of AFs in olive, peanut, and sesame oils in spiked samples (levels between 2.0 and 20.0 microg/kg) ranged from 84 to 92%. The recoveries for AFs B1, B2, G1, and G2 were 86-93, 89-95, 85-97, and 76-85%, respectively. Within-laboratory RSD (RSDr) values for AFs ranged from 3.4 to 10.2%. RSDr values forAF B1, B2, G1, and G2 were 3.5-10.9, 3.2-9.5, 6.5-14.9, and 4.8-14.2%, respectively. Between-laboratory RSD (RSDR) values for AFs were 6.1-14.5%. RSD, values for AFs B1, B2, G1, and G2 were 7.5-15.4, 7.1-14.6, 10.8-18.1, and 7.6-23.7%, respectively. Horwitz ratio values were < or =2 for the analytes in the three matrixes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Aflatoxin B2
Sigma-Aldrich
Aflatoxin B1 from Aspergillus flavus, from Aspergillus flavus
Supelco
Aflatoxin B2
Sigma-Aldrich
Sesame oil from Sesamum indicum, tested according to Ph. Eur.
Supelco
Aflatoxin B2 solution, 0.5 μg/mL in acetonitrile, analytical standard
Supelco
Aflatoxin G2 solution, 0.5 μg/mL in acetonitrile, analytical standard
Supelco
Aflatoxin G1 solution, 2 μg/mL in acetonitrile, analytical standard
Aflatoxin B2 solution, 3.80 μg/g in acetonitrile, ERM®, certified reference material
Aflatoxin G1 solution, 3.78 μg/g in acetonitrile, ERM®, certified reference material
Aflatoxin G2 solution, 3.80 μg/g in acetonitrile, ERM®, certified reference material
Sigma-Aldrich
Sesame oil, Antioxidant, delivery vehicle for fat-soluble compounds
Sigma-Aldrich
Aflatoxin G1, from Aspergillus flavus
Sigma-Aldrich
Olive oil, highly refined, low acidity
Supelco
Olive oil, analytical standard
Sigma-Aldrich
Olive oil, tested according to Ph. Eur.
Aflatoxin B1 solution, 3.79 μg/g in acetonitrile, ERM®, certified reference material
Sigma-Aldrich
Aflatoxin G2
Supelco
Aflatoxin G1
Supelco
Aflatoxin B1 solution, 2 μg/mL in acetonitrile, analytical standard