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  • Degradation, residual determination, and cholinesterase activity of triclorfon in Piaractus mesopotamicus Holmberg (PACU) 1887.

Degradation, residual determination, and cholinesterase activity of triclorfon in Piaractus mesopotamicus Holmberg (PACU) 1887.

Journal of toxicology and environmental health. Part A (2014-02-22)
Maria Isabel Mataqueiro, Laura Satiko Okada Nakaghi, Patrícia Kii Yamada, Guilherme de Camargo Ferraz, Antonio de Queiroz-Neto, Georgino Honorato DeOliveira
ABSTRACT

The aim of this study was to determine the no-observable-adverse-effect concentration (NOAEC) for trichlorfon, an antiparasitic agent used in aquaculture, in Piractus mesopotamicus (pacu) using acetylcholinesterase (AChE) activity as an end point. Fish were exposed 24 h/d for 15 d to different concentrations of trichlorfon in tanks of water for which a curve of dissipation was previously determined. Analysis of trichlorfon in water and fish plasma using gas chromatography with electron capture detection (GC-ECD) enabled measurement of limit of detection (LOD) and limit of quantification (LOQ), respectively, to be 3 and 10 ppb. Thirty-six hours after trichlorfon dilution in water, the concentration was below the LOD, and data showed that plasma concentrations did not exceed the LOQ. Apart from the 6.25 μg/L, all concentrations of trichlorfon significantly inhibited plasma and brain AChE activity compared to controls. The AChE activity levels returned to control values in 7 d. These data may be useful to determine the concentration of trichlorfon that destroys parasites without producing adverse effects in fish.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetylcholinesterase human, recombinant, expressed in HEK 293 cells, lyophilized powder, ≥1,000 units/mg protein (Lowry)
Sigma-Aldrich
Acetylcholinesterase from human erythrocytes, buffered aqueous solution, ≥500 units/mg protein (BCA)
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Acetylcholinesterase from Electrophorus electricus (electric eel), Type V-S, lyophilized powder, ≥1,000 units/mg protein
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Acetylcholinesterase from Electrophorus electricus (electric eel), Type VI-S, lyophilized powder, 200-1,000 units/mg protein