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C2888

Sigma-Aldrich

Acetylcholinesterase from Electrophorus electricus (electric eel)

Type V-S, lyophilized powder, ≥1,000 units/mg protein

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Synonym(s):
AChE, Acetylcholine acetylhydrolase, Cholinesterase, Acetyl
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.32

biological source

Electrophorus electricus

Quality Level

type

Type V-S

form

lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

280 kDa

composition

Protein, ≥45% biuret

pI 

~5.3

solubility

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

storage temp.

−20°C

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This Item
C1682C3389C7512
vibrant-m

C1682

Acetylcholinesterase human

specific activity

≥1,000 units/mg protein

specific activity

≥1,000 units/mg protein (Lowry)

specific activity

200-1,000 units/mg protein

specific activity

≥10 units/mg protein

mol wt

280 kDa

mol wt

64.6 kDa

mol wt

280 kDa

mol wt

tetramer 440 kDa

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

Tris buffer: 1 mg/mL (0.02 M Tris buffer, pH 7.5)

solubility

-

solubility

20 mM Tris HCl buffer, pH 7.5: soluble 1.0 mg/mL, clear(lit.)

solubility

cold water: 60 U/mL

General description

Molecular Weight: 280 kDa
Isoelectric Point: 5.5
Extinction Coefficient: E1% = 18.0 (280 nm)

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

Application

Acetylcholinesterase from Electrophorus electricus (electric eel) has been used:
  • in acetylcholinesterase activity assay
  • to study its effects on Xenopus embryonic hippocampal cells
  • to study the effect of acetylcholine inhibitors on development and plasticity

Biochem/physiol Actions

Acetylcholinesterase (AChE) exhibits its activity against acetylcholine in the neuromuscular junctions and synaptic cleft of cholinergic neurons. It participates in the nitric oxide signaling pathway.
Major degradative enzyme for acetylcholine in vivo. Converts acetylcholine + H2O to choline + acetic acid.

Caution

For dilute solutions (<1 mg/ml), add 1 mg/ml BSA to stabilize. Solutions containing at least 1 mg protein/ml in dilute neutral phosphate buffer are stable at least 6 months refrigerated.

Unit Definition

One unit will hydrolyze 1.0 μmole of acetylcholine to choline and acetate per min at pH 8.0 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts

Analysis Note

The activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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